SunShell
の基本特性
Core shell silica
Bonded phase
Particle size
(
µ
m)
Pore diameter
(nm)
Specific surface
area (m
2
/g)
Carbon content
(%)
Bonded phase
Maximum operating
pressure
Available pH
range
SunShell C18 2.6
9
150
7
C18
60 MPa or 8,570 psi
1.5 - 10
SunShell C8
2.6
9
150
4.5
C8
60 MPa or 8,570 psi
1.5 - 9
SunShell PFP
2.6
9
150
4.5
Pentafluorophenyl
60 MPa or 8,570 psi
2 - 8
0 2 4 6 8 10 12 14 16 18 20 22 24
Retention time/min
Column:
SunShell PFP, 2.6
µ
m 150 x 4.6 mm
SunShell C8, 2.6
µ
m 150 x 4.6 mm
SunShell C18, 2.6
µ
m 150 x 4.6 mm
Mobile phase: CH
3
OH/H
2
O=75/25
Flow rate: 1.0 mL/min
Temperature: 40
º
C
Sample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene
5 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene
Retention of standard samples was compared for three kinds of phases such as C18, C8 and PFP. C18 showed the highest
Hydrogen bonding
(Caffeine/Phenol)
Hydrophobicity
(Amylbenzene/Butylbenzene)
Steric selectivity
(Triphenylene/o-Terphenyl)
SunShell PFP
1.00
1.31
2.38
SunShell C8
0.32
1.46
1.08
SunShell C18
0.39
1.60
1.46
SunShell PFP
SunShell C8
SunShell C18
1
1
1
2
2
7
3
2,3
3
4
4
4
5
5
5
6
6
6
7
7
SunShell C8, PFP
(Pentafluorophenyl)
◆
Characteristics of SunShell
◆
Comparison of standard samples
hydrophobicity and PFP showed both the highest steric selectivity and the highest hydrogen bonding. The feature of PFP phase is
to have hydrogen bonding, dipole-dipole interaction, aromatic and pi-pi interactions and hydrophobicity, which causes a different
selectivity from a C18 phase.
Separation of amitriptyline using C8 Separation of xanthines
Separation of isomers of xylene
SunShell C8 showed a sharp peak for
amitriptyline a well as SunShell C18.
SunShell PFP can retain xanthines more than
SunShell C18. The higher the concentration of
er, the l
.
SunShell PFP showed the different selectivity
from SunShell C18.
0 1
2
3
4
5
Retention time/min
SunShell C18, PFP 2.6
µ
m 150 x 2.1 mm
Mobile phase: CH
3
OH/water or buffer=30/70
Flow rate: 0.3 mL/min
Temperature: 25
º
C
Detection: UV@250nm
Sample: 1 = Theobromine
2 = Theophyline
3 = Caffeine
4 = Phenol
SunShell C8, 2.6
µ
m 150 x 4.6 mm
Mobile phase:
CH
3
CN/20mMphosphate buffer H7.0=60/40
Flow rate: 1.0 mL/min
Temperature: 40
º
C
Detection: UV@250nm
Sample: 1 = Uracil
2 = Propranolol
3 = Nortriptyline
4 = Amitriptyline
1 2
3
4
0 1
2
3
4
5
Retention time/min
SunShell
C18
SunShell
PFP
SunShell
PFP
SunShell
PFP
Water
Water
20mM
phosphate
buffer pH7
50mM
phosphate
buffer pH7
1
2
3
4
1
2
3
4
1 2
3
4
1
2
3
4
SunShell C18, PFP 2.6
µ
m 150 x 2.1 mm
Mobile phase:
CH
3
OH/water=75:25 for SunShell C18
CH
3
OH/water=60:40 for SunShell PFP
Flow rate: 0.3 mL/min
Temperature: 25
º
C
Detection: UV@250nm
Sample: 1 = o-Xylene
2 = m-Xylene
3 = p-Xylene
3
0 1
2
3
4
5
Retention time/min
SunShell C18
SunShell PFP
1
2
1
2
3
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E-mail :
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