★
Sunniest C18, RP-AQUA, C8 are inert for a metal chelating compound and acidic and basic
compounds, so that they show symmetrical peaks of each compound.
Sunniest C18
Sunniest RP-AQUA
Sunniest C8
Column: Sunniest C18, 5
μ
m 150 x 4.6 mm
Sunniest RP-AQUA,
5μ
m 150 x 4.6 mm
Sunniest C8, 5
μ
m 150 x 4.6 mm
Mobile phase: CH
3
CN/20mM H
3
PO
4
=10/90
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = 8-Quinolinol
2 = Caffeine
0
2
4
6
8
10
12
Retention time/min
1
2
2
1
Sunniest RP-AQUA
Sunniest C18
2
1
Sunniest C8
Column: Sunniest C18, 5
μ
m 150 x 4.6 mm
Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Sunniest C8, 5
μ
m 150 x 4.6 mm
Mobile phase: CH
3
CN/0.1% H
3
PO
4
=2/98
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@210nm
Sample: 1 = Formic acid
2 = Acetic acid
3 = Propionic Acid
1
Sunniest C18
Sunniest RP-AQUA
0
2
4
6
8
Retention time/min
3
2
1 2
3
Sunniest C8
2 1
3
◆
Separation of a chelating compound
◆
Separation of acidic compounds
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