C18 RP-AQUA C8
Hydrophobicity
：
α
（
Amylbenzene/Butylbenzene
）
1.56 1.56 1.43
Hydrogen bonding capacity
：
α
（
Caffeine/Phenol
）
0.43 0.49 0.33
Steric selectivity
：
α
（
Triphenylene/o-Terphenyl
）
1.37 1.36 1.23
4
5
6
7
1
3
2
Sunniest C18
0
5
10
15
20
25
30
35
Retention time/min
1
3
4
5
6
7
Sunniest RP-AQUA
Sunniest C8
7
6
5
4
3
2
1
Column: Sunniest C18, 5
μ
m 150 x 4.6 mm
Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Sunniest C8, 5
μ
m 150 x 4.6 mm
Mobile phase: CH
3
OH/H
2
O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Pressure: 5.4 MPa
Sample: 1 = Uracil,
2 = Caffeine,
3 = Phenol,
4 = Butylbenzene, (N=15,700)
5 = o-Terphenyl, (N=15,000)
6 = Amylbenzene, (N=15,300)
7 = Triphenylene, (N=14,500)
2
Column: Sunniest C18, 5
μ
m 150 x 4.6 mm
Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Sunniest C8, 5
μ
m 150 x 4.6 mm
Mobile phase: CH
3
OH/H
2
O=30/70
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250nm
Sample: 1 = Uracil
2 = Pyridine
3 = Phenol
3
2
1
0
2
4
6
8
Retention time/min
Sunniest C18
Sunniest RP-AQUA
1
2
3
Sunniest C8
1
2
3
Sunniest C18
Sunniest RP-AQUA
Sunniest C8
◆
Separation of standard samples
◆
Separation of pyridine and phenol
α
（
Pyridine/Phenol
）
= 0.38
α
（
Pyridine/Phenol
）
= 0.40
α
（
Pyridine/Phenol
）
= 0.41
Separation factor of pyridine and phenol is said to show the
amount of residual silanol groups. The lower a value of
separation factor, the less an effect of residual silanol groups.
All Sunniest columns show one of the lowest value.
N is plate number of the above Sunniest C18.
Sunniest C18, RP-AQUA and C8 show not only high
efficiency but also low column pressure.
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