The EDIT-CHANNELS-DETAILS
Screen (continued)
Setting Up Gradients for Liquid
Chromatography
Data System users may wish to
use the
NO
Board DACs for setting up
an HPLC solvent gradient. PeakSim–
pIe forWindows allows the user to con–
trol the flow of two pumps, provided
they operate from a zero to five volt (0–
5V) ramp input.
To operate the pumps, several in–
ternal connections must be made be–
tween the HPLC and the Data System.
Unplug the Data System and remove
the two screws which secure the top
cover. Route the Pump A and Pump B
control wires from the HPLC to the
Data System and connect the Pump A
control wire to TP1 and the Pump B
control wire to TP2. Re-attach the top
cover, connect power and re-establish
communication between the Data Sys–
tem and the computer.
-
~
FOUR CHANNEL
MODEL 202AlDBOARD
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MODEL 203 AlDBOARD
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Set up the Gradient ramp on
channel one (TP1) to control the flow
of Pump A into the system (1OmV
I
%)
and the Gradient ramp on channel
two (TP2) to control the flow of Pump
B. Modifying the Gradient ramp pro–
gramonChannel 1 to rise from 10% to
90%will automatically create a Gradi–
ent ramp program on Channel 2 that
decreases proportionately from 90% to
10%.
Gradient Limits Zero and Span
may be scaled in the Edit-Overall
screen to account for any offsets.
PeakSimpIe allows for a voltage offset
and scaling factor in these fields to cali–
brate the voltage output to match the
pump's requirements.
