The EDIT-eHANNELS-eOMPONENTS Screen (continued)
TheCalibrate Button
After creabng
iii
component table, each component
in
the
table ......n need to
be
cali–
braled This allows PeakS,,"pIe
fOf
Wmows to
nol only
ilSenbfy each analy1e peak.
but also to quantify each peak using a callbratlOfl curve
The
calibratlOfl curve is cal–
culated
from
user-generated results obtained at
5eVeI<I1
different concentrations that
span
the
expected range to
be
encounlered In
actual
samples.
Inject a standard containing a known conc:entratiClfl of the component you wish
10
calibrate. Use a ecoceoneuco htgher than what you would expect to encounter in your
analyses. Another few samples should
be
run al lower levels, using precise dilutions
of your standard Make note of the area counts or peak
he~ht
at each concentration
Of
use the shortcut method described in the
next
section.
The
CalibrationWindow
..
.–
.–
••••••
,.
iW
••
,.
.
–
.
••
I'
-
-
'-
"
'-
=
In t he Ed lt -Channel s -
Components screen. highlight
the
component
to
be
cal ibrated and
select
Calibnlte
If
this is
the
first
time
cali–
brating a compooent, an error mes–
sage
wil
appear wtlicIl says -Not
enough
d<lta
points- , ThIS is
simpty
a
waming
to
inform
you that PeakSirnple
currently
does
nol
have enough data
points fOf
the
calibration method in
use.
Once
enough data is entered for
the
calibration
curve, irns
message
wi ll
_J~::.cJ!:~~~r;.;;J:=~~~~~
no
longer appear. Click OK to bypass
lhe error message and continue to the
calibration window
/ 0
;_=J:I
__41
'f ....
~
.
.
_ I
-
I
.. ...
-
-
-
-------
--
-
-
-
'0-
...
-
The
Calibration
window
will open
and allow you
10
enler
the
raw data
thai you previously obtained In
the
ex–
ample sOOwn,
data is
entered into
the /
~I-:
: :
table
in
the
upper left comer
of
the
cali-
-
a-
brabon
screen. beg.nmng
with
the
Iowrt-
:f;....
~=-
est
C(ln(;entJaliOn and ending
with
the
highest
eoocereatco.
If
you
wish
10
enter
the
data ., descer'Khng order.
,/
check
the
Show Ii. t in desc.."ding /
~
/
order
box.
When
entenng data into
the
table,
fi~t
enter the concentration
height obtained
