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2
Table of Contents
Overview ............................. 2
ExtractionMethods for Liquid,
Solid, and Biota Samples..... 3
•Liquid Samples
• Separatory Funnel
Extraction
• Liquid-Liquid Extraction
• Solid Phase Extraction
•Soil Samples
• Sonication or Soxhlet
Extraction
• Solvent Selection
Sample CleanupMethods .... 6
•Sulfur and Lipid
Contaminants: Gel
Permeation Chromatography
•Polar Contaminants and
Co-Extractants: Adsorbent
SPE Tubes
•Double-Bond, Triple-Bond, or
Aromatic CompoundCon-
tamination: Sulfuric Acid
Cleanup
•General Contaminants:
Carbon Cleanup
•Sulfur Contamination:
Mercury, ActivatedCopper
Powder Cleanup
Analysis of Chlorinated
Pesticides............................ 9
•Calibration
• Injection Port Maintenance
•Cold On-Column Injections
•Direct Injections
•Split/Splitless Injections
•ResolutionDiscussion
Summary .......................... 14
Product Listings................ 18
he analysis of chlorinated pesticides (as stated inUSEnvironmental
ProtectionAgency (EPA)Methods8081, 508, and608) andpolychlori-
T
natedbiphenyls (PCBs) (asstated inUSEPAMethods8081and8082), are
someof themost common testsperformedby environmental laboratories.
However,many laboratoriesstrugglewith thembecause thesamplesoften
are highly contaminated with non-target compounds such as lipids and
hydrocarbons, and themethods require rigorous quality control. Several
techniques, used in combinationwithRestek’sRtx
®
-CLPesticides column
andRtx
®
-CLPesticides2 column, canhelpyoumore simplyperform these
analyses.
Thecompoundsaddressed in thesemethodsare listed inTable I. The table
includes additional compounds typically analyzed using the samemeth-
ods. Herbicide compounds (USEPAMethod8151) also are listedbecause
many laboratoriesuse thesame instrument toanalyzebothpesticidesand
herbicides.Although theseparationofherbicides is included in thisguide,
the extraction of them, which is significantly different than for pesticides,
is not included. If you are involved in the preparation and analysis of the
herbicidesandwould likemore information, please contactRestek’s tech-
nical service.
Table I
US EPA 608
aldrin
Aroclor
®
1016*
Aroclor
®
1221*
Aroclor
®
1232*
Aroclor
®
1242*
Aroclor
®
1248*
Aroclor
®
1254*
Aroclor
®
1260*
α
-BHC (
α
-HCH)
β
-BHC (
β
-HCH)
δ
-BHC (
δ
-HCH)
γ
-BHC (
γ
-HCH, lindane)
4,4'-DDD
4,4'-DDE
4,4'-DDT
dieldrin
endosulfan I
endosulfan II
endosulfan sulfate
endrin
endrin aldehyde
endrin ketone
heptachlor
heptachlor epoxide
technical chlordane*
toxaphene*
US EPA 8081
aldrin
Aroclor
®
1016*
Aroclor
®
1221*
Aroclor
®
1232*
Aroclor
®
1242*
Aroclor
®
1248*
Aroclor
®
1254*
Aroclor
®
1260*
4,4'-DDD
4,4'-DDE
4,4'-DDT
α
-BHC (
α
-HCH)
β
-BHC (
β
-HCH)
δ
-BHC (
δ
-HCH)
γ
-BHC (
γ
-HCH, lindane)
α
-chlordane
γ
-chlordane
dieldrin
endosulfan I
endosulfan II
endosulfan sulfate
endrin
endrin aldehyde
endrin ketone
heptachlor
heptachlor epoxide
methoxychlor
technical chlordane*
toxaphene*
○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○
○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○
*Multi-component standards.
chlorneb
chlorobenzilate
chlorothalonil
DCPA
4,4'-DDD
4,4'-DDE
4,4'-DDT
dieldrin
endosulfan I
endosulfan II
endosulfan sulfate
endrin
endrin aldehyde
endrin ketone
etridiazole
heptachlor
heptachlor epoxide
hexachlorobenzene
methoxychlor
cis-permethrin
trans-permethrin
propachlor
technical chlordane*
trifluralin
toxaphene*
aldrin
Aroclor
®
1016*
Aroclor
®
1221*
Aroclor
®
1232*
Aroclor
®
1242*
Aroclor
®
1248*
Aroclor
®
1254*
Aroclor
®
1260*
α
-BHC (
α
-HCH)
β
-BHC (
β
-HCH)
δ
-BHC (
δ
-HCH)
γ
-BHC (
γ
-HCH, lindane)
α
-chlordane
γ
-chlordane
US EPA 508
✶
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