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10
Figure 9
FAMEWAX
™
ColumnsMeet the ResolutionCriteria of AOCS andAOAC
Methods andCut Analysis TimeByUp To 50%
30m, 0.25mm ID, 0.25µmFamewax
™
(cat.#12497).
0.8µl split injection ofmenhaden oil PUFAwithC23:0 (IS).
On-column concentration:
100-150ng.
Oven temp.:
120°C to 220°C@7°C/min. (hold20min.);
Inj./det. temp.:
220°C;
Carrier gas:
hydrogen;
Linear velocity:
60cm/sec. @120°C;
FID sensitivity:
8 x 10
-11
AFS
Split ratio:
50:1.
min.
8
10
12
14
16
18
20
22
30
32
31
36
35
37
18
19 20
21
22
FAMEWAX
™
columnsmeet all the criteria listed in themethods in significantly less time, with faster flow and tempera-
ture program rates than other Carbowax
®
columns. The menhaden oil PUFA analysis on a FAMEWAX
™
column in
Figure9
shows thatC21:5n3andC23:0 (IS) arewell resolved, asareC24:0, C22:6n3 (DHA), andC24:1n9,witha total
analysis time of only 22minutes.
The same analysis on another PEG column typicallyused for FAMEs analysis shows
peaks C21:5n3 and C23:0 are not baseline resolved, nor are C22:6n3 and C24:1n9. To achieve resolution of these
components on this other column, the program rate must be decreased to 2°C or 3°C/minutes, which increases the
analysis time by 59%!
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31
30
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37
32
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10
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13
14
15
16
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19
20
21 22
23
24
25
26
27
28
1
2 3
4
5
6
7
8
34
33
29
For peak identifications, please
refer to Figure 10.
33
35
