ADV06-15_1-412

412 / 413

Comprehensive 20 Gas Chromatography ­
Making GCSeparations Work Harder Continued from page 2.
deconvoluti on. GC deconvolution through real separation is a more rugged and desirable
outcome - and we can still combine GCxGC with MS for further identification. It is cer­
tainly true that GCxGC demands improved performance capabilities of GC instru men ts,
new software, and better column quality contro l (e.g., imp roved batch-to-batch column
repro ducibility for the I m length s of 2D columns that we use). These cannot be realised
without the compliance of inst rument and column manufacturers. As examples of generic
GCxGC applications, a low polarity (5% phenyl) ID column coupled to a short polar (wax
phase) 2D column is useful for essent ial oils, but recently wax-low polarity column combi­
nations have proved equally valuable. For petrochemicals, where higher temp erature oper­
ation is needed, a low polarity (5% phenyl) ID column coupled to a short polar (50%
phenyl) 2D column is often used. For environ mental analysis of PCBs, a carborane phase 2D
column has been repo rted, where selectivity towards the extent of compoun d planarit y is
sought. In this shor t commentary, there is no space to engage in specifics of certain GCxGC
methods, but obviously there is considerab le opportunity to optimise methods, and use
sound principles of GC and phase selection to get the best out of GCxGc.
We are at the th reshold of a new era in GC, and getting the best out of our GCxGC meth­
ods is a task that an increasing number of analysts wiII be striving for. The comments of
Professor Walt Jenni ngs (Restek Advantage, 2006.01) also ring tru e for GCxGc. When a
method has been developed for GCxGC, and one of the columns has to be replaced, to what
extent will the 2D plot faithfully reproduce our archived or master analytical result? This
must be addressed in the two-dimensiona l experiment, to prove that the analyst can have
confidence that their data interpretation protocols survive column change and routine
maintenance of the system. But with the impressive capabilities of GCxGC , it is impor tant
that analytical methods and the greater informatio n content it offers are supported by vali­
dated and reliable operation.
Editor's note: Dr. Marriott is one of the world's leading experts in 2D-GC.
An Example of 20 GasChromatography see Advantage 2005.1
GCxGC analysis of org anochl orine
pesticides combines prim ary
column and confirmation column
results.
Organochl orine pesticides
separated from interferences in
tomato ext ract.
Columns: Rtx~-5 9m, O.18mm 10,
0.20l' m(10m column, cal.#
40201, with 1mremoved)
Rtx°-200lm,O.l8mm 10,
0.201'm (Im of10mcolumn,
cal.# 45001)
In].: !Ill, split, 250"C, splitratio
50:1
Oven: Primary: 50"C (0.2 min.),
30' C/min. to140' (no hold),
5"C/min. to 25O"C (no hold)
Secondary:50' ( offset from
primary oven
Instrument: lECOGCxGC/ECD
Modulator: Temperature offset: 30"C
Modulationtime:6 sec
Del.: ECD, 325'C, 150mllmin.
nitrogenmakeupgas, 50Hz
Tradeshow Schedule
We'd be happy to talk with you at any of the
following meetings or 'll post our
booth numbers asthey become available to us.
September, 2006
Date September 17-21
Show 120th AOAC Annual
Meet ing & Exposition
Location Hyatt Regency, Minneapolis, MN
October, 2006
Date October 3-7
Show SOFT2006 Annual Meet ing
Locatio n Hilton Austin, Austin, TX
Date October 7-10
Show ACIL 69th Annual Meeting
Location San Antonio Marriott River Center,
San Antonio, TX
Date October 11
Show Chromatography Society
Triad Symposia
Location AstraZeneca, Charnwood, England
Date October 11-12
Show Midwestern Association of Forensic
Scientists (MAFS)
Location Hyatt downtown, Indianapolis, IN
Date October 17-19
Show Gulf Coast Conference
Location Moody Gardens Convention Center
& Hotel, Galveston Island, TX
Date October 24-25
Show Chromatography Society
Triad Symposia
Location Pfizer, Sandwich , England
Date October 31 - November 3
Show SEMAShow
Location LasVegas Convention Center,
LasVegas, NV
November, 2006
Date November 1-2
Show WWEMWater, Wastewater and
Environmental Monitoring
Conference Exhibition & Workshops
Location Telford International Centre, Telford,
Shropshire, England
Date November 1-4
Show 32nd Annual NEAFS Meeting
Location Tarrytown DoubleTree Hotel,
Tarrytown, NY
Date November 6-7
Show California Association
ofToxicologists
Location Miramonte Resort and Spa,
Palm Springs, CA
Date November 6-10
Show SWAFS/NWAFS Joint Meeting
and Training Conference
Location Doubletree Hotel,ColoradoSprings,CO
Date November 9
Show 2006 Anachem Symposium
Location Burton Manor, 27777
Schoolcraft Rd., Livonia, MI
Date November 13-16
Show 2006 Eastern Analytical Symposium
Location Garden State Exhibit Center,
Somerset, NJ
Date November 21-22
Show Chromatography Society
Triad Symposia
Location GlaxoSmithKline, Stevenage, England
For latest updates, see ourTradeshow Calendar
at
Website : E-mail : TelNo : 03 9762 2034 . . . in AUSTRALIA