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Pharmaceutical
Figure 2 An Ultra Aqueous (18 column shows suitable retent ion
for polar or nonpolar compounds, providing enhanced selectivity
for a broad range of polarity.
A) Ultra Aqueous C18 column
Best balance of for more polar and less polar analytes
5 7
i ~ i I
10
Min.
LC PH0400
B) Conventional C18 column
Lessretention and resolution of polar compounds
45 7
I
10
Min.
I..C_PH0399
C) Intrinsically base deactivated (lBO) phase
Poor retention of less polar compounds
2
1. 3,5-dihydroxybenzoic acid
2. 2,5-dihydroxybenzoic acid
3. 4-hydroxybenzoicacid
4. 3-hydroxybenzoic acid
5. 2,4-dihydroxybenzoic acid
6. benzoicacid
7. salicylic acid
sample:
Inj.: Sill
Cone.: 50llg/ ml eachcomponent
Samplediluent: mobile phase
Column: UltraAqueous CI8 (cat# 9178568)
Dimensions: 150 x 4.6mm
Particle size: 511"1
Pore size: 100A
Conditions:
Mobile phase: A: 20mMpotassiumphosphate (pH2.5); B: acetonitrile
Time
(min.) %B
0.00 20
5.00 50
10.00 50
Flow: 1.OmLlmin.
Temp.: ambient
Det.: UV @210om
More polar
compounds
Best resolution, P
(Ik Lesspolar
compounds
6
Min.
I..Cj H0401
2006.03 • 19 •
It is well documented that Ultra Aqueous CIS
columns are compatible with 100% aqueous
mobile phases, because the stationary phase has
sufficient polar character to prevent dewetting or
hydroph obic collapse.' Our cur rent analyses reveal
yet another advantage to the slight polar character
of this column: by providing the best resolution of
analytes exhibiting a wide range of polarity, the
Ultra Aqueous CIS column demonstrates that it
also can be used to retain, and separate, more polar
or less polar comp ounds - or mixtures of both .
Reference
1 Ultra Aqueous C18 HPLCColumns: AchieveStable Retentionin100%
Aqueous MobilePhase RestekCorporation, 2002(lit. cat.# 59371).
Referenceavailable on request.
*The intrinsically basedeactivated(IBD)phaseshowsincreased reten­
tion for polar compounds, becausetheembeddedpolar groups arecapa­
bleof interaction with polar portions of analytemolecules.
Ultra Aqueous C18 Columns
3tlmColumn. 2.1mm cat # price
30mm 9178332 $344
50mm 9178352 $344
100mm 9178312 $370
3tlmColumn. 3.2mm cat # ~
30mm 91783lL~)~
50mm 9178353 $344
100mm 9178313 $370
3tlmColumn. 4.6mm cat # rice
30mm 9178335 $344
50mm 9178355 $344
100mm 9178315 $370
~m Column,1.1mm cat # IlriCe
30mm 9178532 $319
50mm 9178552 $319
100mm 9178512 $344
150mm 9178562 $370
200mm 9178522 $396
250mm 9178572 $423
5/..Im Column. 3.2mm C{l~~
30mm 9178533 $319
50mm 9178553 $319
100mm 9178513 $344
IS0mm 9178563 $370
200mm 9178523 $396
250mm 9178573 $423 ,
SpmColumn. 4.6mm cat # price /
30mm 9178535 $319
50mm 9178555 $319
100mm 9178515 $344
150mm 9178565 / $370
200mm 9178525 $396
250mm 91785751'$423
All columns alsoavailable withI rident! integratedguard'column
configuration. call for more details. ~
800-356-1688 •
II
Website : E-mail : TelNo : 03 9762 2034 . . . in AUSTRALIA