ADV01-05_1-298

• 15 •

800-356-1688 •

www.restek.com

2005 vol. 1

New Rtx®-440 GC Column, for Rapid Analysis

of Pesticides, PAHs or Other Semivolatiles

Exclusive Stationary Phase from Restek

by Gary Stidsen, GC Columns Marketing Manager

•Unique selectivity compared to other phases.

• Ideal polarity for pesticides, many other semivolatile compounds.

• Low bleed, thermally stable to 340ºC—excellent for trace analysis by GC/MS.

New Rtx

-440 stationary phase exhibits unique

selectivity at an intermediate polarity.

Applications testing we have performed to date

includes organochlorine pesticides, polycyclic

aromatic hydrocarbons (PAHs), and semivolatile

environmental compounds (US EPA Method

8270). The new column resolves 20 commonly

monitored organochlorine pesticides in 10 min-

utes (Figure 1), or the 34 organochlorine pesti-

cides listed in US EPA Method 8081 in less than

13 minutes.

We also have had very promising results with

analyzing PAHs on Rtx

-440 columns, as shown

in Figure 2. Phenanthrene and anthracene

(peaks 5 and 6), benzo(a)anthracene and chry-

sene (peaks 9 and 10) and benzo(b)fluoranthene

and benzo(k)fluoranthene (peaks 11 and 12) are

resolved well, as is a more difficult pair of ana-

lytes: indeno(1,2,3-cd)pyrene and

dibenzo(a,h)anthracene (peaks 14 and 15). The

analysis is complete in less than 18 minutes.

Our chemists are continuing their applications

research with this new phase. Please call our

Technical Service team to obtain the latest infor-

mation about Rtx

-440 columns.

Figure 1

Separate 20 organochlorine pesticides in 10 minutes, using an Rtx®-440 column.

Column:

Rtx

-440 30m, 0.25mm ID, 0.25µm (cat.# 12923)

Sample:

610 PAH Mix (cat.# 31011) diluted to 20ppm each compound in methylene chloride

Inj.:

1.0µL splitless (hold 0.4 min.), 4mm splitless liner (cat.# 20772)

Inj. temp.:

320°C

Carrier gas:

hydrogen, constant flow

Flow:

3.6mL/min.

Oven temp.:

40°C (hold 2 min.) to 240°C @ 30°C/min., to 320°C @ 8°C/min. (hold 5 min.)

Det.:

FID @ 320°C

1. naphthalene

2. acenaphthylene

3. acenaphthene

4. fluorene

5. phenanthrene

6. anthracene

7. fluoranthene

8. pyrene

9. benzo(a)anthracene

10. chrysene

11. benzo(b)fluoranthene

12. benzo(k)fluoranthene

13. benzo(a)pyrene

14. indeno(1,2,3-cd)pyrene

15. dibenzo(a,h)anthracene

16. benzo(ghi)perylene

Figure 2

Analyze 16 PAHs in 22 minutes, and resolve critical pairs, with an Rtx®-440 column.

GC_EV00732

Rtx

-440 Columns (fused silica)

(proprietary intermediate-polarity Crossbond

phase)

Temp. limits: 20°C to 320/340°C

df (µm)

30-Meter

0.25mm

0.25

12923

$445

0.50

12938

$445

0.32mm

0.25

12924

$470

0.50

12939

$470

0.53mm

0.50

12940

$525

1.00

12955

$525

critical pairs

well resolved

new

aldrin

-BHC

-BHC (lindane)

-chlordane

4,4'-DDD

4,4'-DDE

4,4'-DDT

dieldrin

endosulfan I

endosulfan II

endosulfan sulfate

endrin

endrin aldehyde

endrin ketone

heptachlor

heptachlor epoxide (B)

methoxychlor

Organochlorine Pesticide Mix AB #1

200µg/mL each in hexane:toluene (1:1), 1mL/ampul

cat. # 32291 (ea.)

$43

decachlorobiphenyl

2,4,5,6-tetrachloro-

-xylene

Pesticide Surrogate Mix

200µg/mL each in acetone, 1mL/ampul

cat. # 32000 (ea.)

$19

acenaphthene

acenaphthylene

anthracene

benzo(a)anthracene

benzo(a)pyrene

benzo(b)fluoranthene

benzo(k)fluoranthene

benzo(ghi)perylene

chrysene

dibenzo(a,h)anthracene

fluoranthene

fluorene

indeno(1,2,3-cd)pyrene

naphthalene

phenanthrene

pyrene

SV Calibration Mix #5 / 610 PAH Mix

2,000µg/mL each in methylene chloride, 1mL/ampul

cat. # 31011 (ea.)

$80

GC_EV00751

Column:

Rtx

-440 30m, 0.32mm ID, 0.50µm (cat.# 12939)

Sample:

50ng/mL Organochlorine Pesticides Mix AB standard (cat#32291) &

50ng/mL Pesticide Surrogate Mix (cat#32000) in hexane

Inj.:

1.0µL splitless (hold 1.0 min.), 3.5mm ID single gooseneck inlet liner (cat.# 20962)

Inj. temp.:

250°C

Carrier gas:

hydrogen, constant pressure

Linear velocity:

71cm/sec. @ 110°C

Oven temp.:

110°C (hold 0.5 min.) to 268°C @30°C/min. to 290°C @ 11°C/min. to 320°C @ 25°C/min. (hold 5 min.)

Det.:

ECD @ 310°C

1. 2,4,5,6-tetrachloro-

-xylene

-BHC

6. heptachlor

7. aldrin

8. heptachlor epoxide

-chlordane

10.

-chlordane

11. endosulfan I

12. 4,4' -DDE

13. dieldrin

14. endrin

15. 4,4' -DDD

16. endosulfan II

17. endrin aldehyde

18. 4,4' -DDT

19. endosulfan sulfate

20. methoxychlor

21. endrin ketone

22. decachlorobiphenyl