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bioaccumulation (e.g., PCB#169). The list encom-
passes the seven European indicator compounds
and the 12 most toxic congeners, according to the
World Health Organization (WHO) list.²
Using a 40m x 0.18mm ID, 0.18µm film Rtx®-PCB
column and the conditions listed in Figure 2, we
evaluated a sample composed of Aroclor® PCB
mixes 1242, 1254, and 1262. The 158 target PCB
congeners were identified in the sample, eluted as
135 chromatographic peaks. Of the 158 congeners,
110 eluted singly and 48 were unresolved.
Produced through one of our newest polymer
technologies, Rtx®-PCB columns undergo rigor-
ous quality assurance measures, to ensure every
column meets exacting standards and to give you
highly reproducible performance, from column to
column. If you are analyzing PCBs—as congeners,
as Aroclor® mixtures, or as other mixtures (e.g.,
Kaneclor, Clophen, or Phenoclor mixes)—we
highly recommend using these new columns.
Table I 110 of 158 target PCB congeners in Aroclor® mixes are eluted
singly from an Rtx®-PCB column.
PCB# s/m*
1 s
2 s
3 s
4 m
5 s
6 s
7 s
8 s
9 s
10 m
11 m
12 m
13 m
15 s
16 s
17 m
18 s
19 s
20 m
21 m
22 s
23 m
24 m
25 s
26 s
27 m
28 s
29 s
31 m
32 s
33 m
34 s
PCB# s/m*
35 s
37 s
40 s
41 s
42 s
43 s
44 s
45 s
46 s
47 s
48 s
49 s
51 m
52 s
53 m
54 m
55 s
56 m
57 m
59 s
60 m
63 s
64 s
66 s
67 s
70 s
71 s
74 s
75 s
76 m
77 m
81 s
PCB# s/m*
82 s
83 s
84 m
85 s
86 m
87 m
88 m
89 m
90 m
91 s
92 s
93 m
94 m
95 m
96 s
97 s
99 m
101 m
102 s
103 s
105 m
109 m
110 s
114 s
115 s
117 m
118 m
119 s
122 s
123 m
124 s
125 m
PCB# s/m*
126 s
128 m
129 s
130 s
131 m
132 s
133 s
134 s
135 s
136 m
137 s
138 s
139 s
141 s
144 s
146 s
147 m
149 m
151 s
153 s
154 m
156 s
157 s
158 s
163 s
164 s
166 s
167 s
169 s
170 s
171 s
172 s
PCB# s/m*
173 s
174 m
175 s
176 m
177 s
178 s
179 s
180 s
183 s
185 s
187 s
189 s
190 m
191 s
193 s
194 s
195 s
196 m
197 s
198 s
199 s
200 s
201 s
202 s
203 s
205 s
206 s
207 s
208 s
209 s
For additional PCB congener mixes, and Aroclor® reference
materials, please see our current catalog, or visit our website.
References
1. Frame, G., J. Cochran, and S., Bowadt, Complete PCB Congener
Distributions for 17 Aroclor Mixtures Determined by 3 HRGC Systems
Optimized for Comprehensive, Quantitative, Congener-Specific
AnalysisJ. High Res. Chromatogr. 19, Dec. 1996, pp. 657-668.
2. Executive Summary, Assessment of the Health Risk of Dioxins:
Re-evaluation of the Tolerable Daily Intake (TDI), WHO Consultation,
May 25-29, 1998, Geneva Switzerland. See:
Figure 2 Excellent separation and peak shape for PCBs in three
Aroclor® mixes, using an Rtx®-PCB column.
2,4,4' (BZ #28)
2,2',5,5' (BZ #52)
2,2',4,5,5' (BZ #101)
2,3',4,4',5 (BZ #118)
2,2',3,4,4',5' (BZ #138)
2,2',4,4',5,5' (BZ #153)
2,2',3,4,4',5,5' (BZ #180)
PCB Congener Standard #2
10µg/mL each in isooctane, 1mL/ampul
cat. # 32294 (ea.) $30
Rtx®-PCB (fused silica)
The maximum temperatures listed are for 15- and 30-meter
lengths. Longer lengths may have a slightly reduced maximum
temperature.
ID df (µm) temp. limits length cat. # price
0.18mm 0.18 30°C to 320/340°C 20-Meter 41302 $360
0.18mm 0.18 30°C to 320/340°C 60-Meter 41304 $470
0.25mm 0.25 30°C to 320/340°C 30-Meter 13223 $445
0.25mm 0.25 30°C to 320/340°C 60-Meter 13226 $470
0.32mm 0.50 30°C to 320/340°C 30-Meter 13239 $470
*s - compound eluted singly; m - compound eluted with one or more other congeners.
Column: Rtx®-PCB 40m, 0.18mm ID, 0.18µm (cat.# 41303)
Sample: 300ng/mL Aroclor® 1242/1254/1262 in hexane: Aroclor® 1242 (cat.# 32009),
Aroclor® 1254 (cat.# 32011), Aroclor® 1262 (cat.# 32409)
Inj.: 1.0µL splitless (hold 0.75 min.), 4mm single gooseneck inlet liner (cat.# 20983)
Inj. temp.: 230°C
Carrier gas: hydrogen, constant pressure
Linear velocity: 40cm/sec. @ 100°C
Oven temp.: 100°C (hold 1 min.) to 200°C @ 30°C/min., to 320°C @ 2°C/min. (hold 1 min.)
Det.: ECD @ 330°C
GC_EV00768
min.
min.