69
GCCOLUMNS |
APPLICATION-SPECIFICCOLUMNS
Foods, Flavors & Fragrances
GC_FF00652
FAMEs (
cis
/
trans
isomers)onanRt®-2560column.
Column:
Rt
®
-2560, 100m, 0.25mm ID, 0.2µm (cat.# 13199)
Sample:
cis
/
trans
FAMEMix (cat.# 35079), 10mg/mL total FAMEs inmethylene chloride
Inj.:
1.0µL split (split ratio 20:1), 4mm inlet liner (cat.# 20814)
Inj. temp.:
225°C
Carrier gas:
hydrogen, constant flow
Flow rate:
1.2mL/min.
Oven temp.:
100°C (4min. hold) to 240°C@ 3°C/min. (10min. hold)
Det.:
FID@ 250°C
Compound
% inMix
1. C18:0methyl stearate
20.0
2. C18:1methyl petroselaidate (
trans
-6)
8.0
3. C18:1methyl elaidate (
trans
-9)
10.0
4. C18:1methyl transvaccenate (
trans
-11)
12.0
5. C18:1methyl petroselinate (
cis
-6)
8.0
6. C18:1methyl oleate (
cis
-9)
10.0
7. C18:1methyl vaccenate (
cis
-11)
12.0
8. C18:2methyl linoleate (
cis
-9,12)
20.0
Excellent resolution
of
cis
&
trans
isomers!
cis
/
trans
FAMEs
similar
phases
SPB-2560, HP-88, Silar 10C, CP-Sil 88FAME,
CP-Sil 88
Rt®-2560Column
(fusedsilica)
(highlypolar phase; biscyanopropyl polysiloxane—not bonded)
• Application-specific column for
cis
/
trans
FAMEs.
• Stable to250 °C.
Because theRt®-2560 stationaryphase is not bonded, it shouldnot be solvent rinsed.
SolutionsForYourFoods, Flavors&FragrancesAnalyses
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ID
df
temp. limits
100-Meter
0.25mm 0.20µm 20 to 250°C 13199
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
