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FAMEs (cis/trans isomers) on Rt®-2560
GC COLUMNS | APPLICATION-SPECIFIC COLUMNS
Foods, Flavors, & Fragrances
FAME Analysis (cis/trans)
32 33 34 35
1
2
4
5
3 6
7
8
36 37 38 39 40 41 42
GC_FF00652
Column Rt®-2560, 100 m, 0.25 mm
ID, 0.20 µm (cat.# 13199)
Sample cis/trans FAME mix
(cat.# 35079)
Diluent: Methylene chloride
Conc.: 10 mg/mL total FAMEs
Injection
Inj. Vol.: 1 µL split (split ratio 20:1)
Liner: Splitless (4 mm ID)
(cat.# 20814)
Inj. Temp.: 225 °C
Oven
Oven Temp.: 100 °C (hold 4 min) to 240 °C
at 3 °C/min (hold 10 min)
Carrier Gas H 2, constant flow
Flow Rate: 1.2 mL/min
Detector FID@ 250 °C
Peaks Conc.
(wt.%)
1. C18:0 methyl stearate 20.0
2. C18:1 methyl petroselaidate (trans -6) 8.0
3. C18:1 methyl elaidate (trans-9) 10.0
4. C18:1 methyl transvaccenate (trans-11) 12.0
5. C18:1 methyl petroselinate (cis-6) 8.0
6. C18:1 methyl oleate (cis-9) 10.0
7. C18:1 methyl vaccenate (cis-11) 12.0
8. C18:2 methyl linoleate (cis-9,12) 20.0
Excellent resolution of
cis & trans isomers!
FAMEs (Marine Oil Standard) on FAMEWAX
FAME Analysis (Polyunsaturated)
GC_FF00499
Column FAMEWAX, 30 m, 0.32 mm ID, 0.25 µm
(cat.# 12498)
Sample Marine oil FAME mix (cat.# 35066)
Diluent: Isooctane
Conc.: 10,000 µg/mL (total FAMEs; see breakdown
in peak list)
Injection
Inj. Vol.: 1 µL split (split ratio 100:1)
Inj. Temp.: 250 °C
Oven
Oven Temp.: 195 °C to 240 °C at 5 °C/min (hold 1 min)
Carrier Gas H 2, constant flow
Flow Rate: 3 mL/min
Detector FID@ 275 °C
Peaks Conc.
(µg/mL)
1. C14:0 600
2. C14:1 100
3. C16:0 1,600
4. C16:1 500
5. C18:0 800
6. C18:1 (oleate) 1,300
7. C18:1 (vaccenate) 400
8. C18:2 200
9. C18:3 200
10. C20:0 100
11. C20:1 900
12. C20:2 100
13. C20:4 300
14. C20:3 100
15. C20:5 1,000
16. C22:0 100
17. C22:1 300
18. C24:0 100
19. C22:6 1,200
20. C24:1 100
Column engineered for
optimal resolution.
FAMEs Analyses
High-Resolution GC Analyses
of Fatty Acid
Methyl Esters
lit. cat.# 59584B
Download your
free copy from
Rt®-2560 Column (fused silica)
(highly polar phase; biscyanopropyl polysiloxane—not bonded)
• Application-specific column for cis/trans FAMEs.
• Stable to 250 °C.
Because the Rt®-2560 stationary phase is not bonded, it should not be solvent rinsed.
similar phases
HP-88, CP-Sil 88, SPB-2560
100-Meter
ID df temp. limits cat.# price
0.25 mm 0.20 µm 20 to 250 °C 13199
FAMEWAX Columns (USP G16) (fused silica)
(polar phase; Crossbond® polyethylene glycol)
• Application-specific columns for FAMEs, specially tested with a FAME mixture.
• Temperature range: 20 °C to 250 °C.
The elution order of polyunsaturated FAMEs on FAMEWAX columns is comparable to
that on other Carbowax® columns, but baseline resolution is achieved in significantly
less time.
similar phases
Select FAME, Omegawax
30-Meter
ID df temp. limits cat.# price
0.25 mm 0.25 µm 20 to 240/250 °C 12497
0.32 mm 0.25 µm 20 to 240/250 °C 12498
0.53 mm 0.50 µm 20 to 250 °C 12499