Rtx015-16-628

67 / 629

65
GC COLUMNS | APPLICATION-SPECIFIC COLUMNS
Clinical, Forensic, & Toxicology
Rtx®-BAC Plus 1 Columns (fused silica)
Rtx®-BAC Plus 1/Rtx®-BAC Plus 2 Columns
• Optimized column selectivities guarantee resolution of ethanol, internal standards,
and frequently encountered interferences.
• Robust and reproducible column chemistry ensures longer column lifetime and
consistent results.
• Stable to 260 °C.
These application-specific columns for blood alcohol analysis baseline separate all
critical compounds—including ethanol, methanol, acetone, tert-butanol, acetalde-
hyde, isopropanol, and n-propanol—in less than 2 minutes. Every Rtx®-BAC Plus 1
and Rtx®-BAC Plus 2 column is qualified with a test mix containing these important
BAC target compounds to ensure reproducibility.
These columns baseline separate all blood alcohol compounds in blood, breath, or
urine, in less than 2 minutes, under isothermal conditions. Isothermal analysis in-
creases productivity by eliminating the need for oven cycling. Confirmation is easily
achieved with this tandem set because there are two elution order changes between
the columns.
Rtx®-BAC Plus 2 Columns (fused silica)
Blood Alcohol Analysis
Blood Alcohol Analysis on Rtx®-BAC Plus 1 and Rtx®-BAC Plus 2
Time (min) 0.5 0.75 1 1.25 1.5 1.75 2 2.25 2.5 2.75
1
2
3 4
5
6
7
Blood Alcohol Analysis on Rtx®-BAC Plus 1
GC_CF1153
Column Rtx®-BAC Plus 1, 30 m, 0.32 mm ID, 1.8 µm (cat.# 18004)
Sample BAC resolution control standard n-P (cat.# 36010)
BAC resolution control standard t-B (cat.# 36011)
Diluent: Water
Conc.: 50 µL of each standard were diluted in 900 µL water in a
20 mL headspace vial.
Injection Headspace-loop split (split ratio 50:1)
Liner: 1 mm ID straight inlet liner (cat.# 20972)
Headspace-Loop
Inj. Port Temp.: 200 °C
Instrument: Tekmar HT3
Inj. Time: 3 min
Transfer Line
Temp.: 125 °C
Valve Oven Temp.: 125 °C
Standby flow
rate: 50 mL/min
Sample Temp.: 60 °C
Sample Equil.
Time: 5 min
Vial Pressure: 30 psi
Pressurize Time: 1 min
Loop Pressure: 20 psi
Loop Fill Time: 1 min
Oven
Oven Temp.: 40 °C (hold 3 min)
Carrier Gas He, constant flow
Linear Velocity: 80 cm/sec @ 40 °C
Detector FID@ 240 °C
Make-up Gas
Flow Rate: 30 mL/min
Make-up Gas
Type: N2
Instrument Agilent/HP6890 GC
Notes Headspace concentrator courtesy of Teledyne Tekmar,
Mason, OH.
Peaks tR (min) Conc.
(µg/mL)
1. Methanol 0.912 100
2. Acetaldehyde 0.967 100
3. Ethanol 1.158 100
4. Isopropanol 1.420 100
5. Acetone 1.548 100
6. tert-Butanol 1.688 50
7. 1-Propanol (n -Propanol) 1.969 50
Time (min) 0.5 0.75 1 1.25 1.5 1.75 2 2.25 2.5 2.75
1
2
3
4
5
6
7
Blood Alcohol Analysis on Rtx®-BAC Plus 2
GC_CF1154
Peaks tR (min) Conc.
(µg/mL)
1. Acetaldehyde 0.809 100
2. Methanol 0.861 100
3. Ethanol 1.034 100
4. Acetone 1.119 100
5. Isopropanol 1.181 100
6. tert-Butanol 1.304 50
7. 1-Propanol (n-Propanol) 1.658 50
free literature
Rtx®-BAC Plus 1 and
Rtx®-BAC Plus 2 Columns
Advanced Technology for Fast,
Reliable Measurement
of Alcohol in Blood
Download your
free copy from
lit. cat.#
CFBR1538-UNV
30-Meter
ID df temp. limits cat.# price
0.32 mm 1.80 µm -20 to 240/260 °C 18004
0.53 mm 3.00 µm -20 to 240/260 °C 18005
30-Meter
ID df temp. limits cat.# price
0.32 mm 0.6 µm -20 to 240/260 °C 18006
0.53 mm 1.0 µm -20 to 240/260 °C 18007
similar phases
DB-ALC1, ZB-BAC1
DB-ALC2, ZB-BAC2