96
www.restek.comOrganic Volatile Impurities on Rtx®-5 (Rtx®-G27)
GC COLUMNS |
APPLICATION-SPECIFIC COLUMNS
Pharmaceutical
Organic Volatile Impurities (OVI) Analysis
G27 phase
USP
Pharmaceutical
Standards
See
pages 595–596 .GC_PH00260
Column
Rtx®-5 w/5m Integra-Guard® Column (Rtx®-G27), 30 m, 0.53 mm ID, 5.00 µm (cat.# 10279-126)
Sample
Headspace injection of common solvents for pharmaceutical processing. Prepared to equal about 500 ppm in the bulk
pharmaceutical. Samples shaken and heated at 90 °C for 15 minutes, 1 mL headspace injection.
Injection
Inj. Vol.:
1,000 µL headspace-syringe split (split ratio 2:1)
Inj. Temp.:
220 °C
Oven
Oven Temp.:
35 °C (hold 10 min) to 100 °C at 5 °C/min to 240 °C at 25 °C/min (hold 5 min)
Carrier Gas
He, constant pressure
Linear Velocity:
35 cm/sec @ 35 °C
Detector
FID@ 240 °C
Notes
FID sensitivity: 1.05 x 10
-11
AFS
Peaks
1. Ethylene oxide
2. Methanol
3. Ethanol
4. Diethyl ether
5. 1,1-Dichloroethene
6. Acetone
7. Isopropanol
8. Acetonitrile
9. Methylene chloride
10.
n
-Hexane
11.
n
-Propanol
12. Methyl ethyl ketone
13. Ethyl acetate
14. Tetrahydrofuran
15. Chloroform
16. 1,1,1-Trichloroethane
17. Carbon tetrachloride
18. Benzene
19. 1,2-Dichloroethane
20. Heptane
21. Trichloroethylene
22.
n
-Butanol
23. 1,4-Dioxane
24. Pyridine
25. Toluene
Similar to: (5%-phenyl)-methylpolysiloxane
Rtx®-5 (G27) Columns
(fused silica)
(low-polarity phase; Crossbond® diphenyl dimethyl polysiloxane)
Rtx®-5 Structure
CH
3
CH
3
Si
95%
O
Si
5%
O
similar phases
HP-5, DB-5, CP-Sil 8 CB, ZB-5
NOTE: DB-5MS is a silarylene-based
polymer, similar to Rxi-5Sil MS.
*Maximum temperatures listed are for shorter length columns. Longer columns may have a different maximum temperature.
• General-purpose columns for drugs, solvent impurities, pesticides, hydrocarbons,
PCB congeners (e.g., Aroclor mixes), essential oils, semivolatiles.
• Temperature range: -60 °C to 350 °C.
• Equivalent to USP G27 and G36 phases.
The diphenyl dimethyl polysiloxane stationary phase is the most popular GC station-
ary phase and is used in a wide variety of applications. All residual catalysts and low
molecular weight fragments are removed from the Rtx®-5 polymer, providing a tight
mono-modal distribution and extremely low bleed.
15-Meter
30-Meter
60-Meter
105-Meter
ID
df
temp. limits* cat.#
price
cat.#
price
cat.#
price
cat.#
price
0.25 mm 0.10 µm -60 to 330/350 °C 10205
10208
10211
0.25 µm -60 to 330/350 °C 10220
10223
10226
10229
0.50 µm -60 to 330/350 °C 10235
10238
10241
10244
1.00 µm -60 to 325/340 °C 10250
10253
10256
10259
0.32 mm 0.10 µm -60 to 330/350 °C 10206
10209
0.25 µm -60 to 330/350 °C 10221
10224
10227
0.50 µm -60 to 330/350 °C 10236
10239
10242
1.00 µm -60 to 325/340 °C 10251
10254
10257
10260
1.50 µm -60 to 310/330 °C 10266
10269
10272
10275
3.00 µm -60 to 280/300 °C 10281
10284
10287
10290
0.53 mm 0.10 µm -60 to 320/340 °C 10207
10210
0.25 µm -60 to 320/340 °C 10222
10225
10228
0.50 µm -60 to 320/330 °C 10237
10240
10243
1.00 µm -60 to 320/330 °C 10252
10255
10258
1.50 µm -60 to 310/330 °C 10267
10270
10273
3.00 µm -60 to 270/290 °C 10282
10285
10288
5.00 µm -60 to 270/290 °C 10277
10279
10283
10-Meter
20-Meter
40-Meter
ID
df
temp. limits
cat.#
price
cat.#
price
cat.#
price
0.18 mm 0.20 µm -60 to 325/340 °C 40201
40202
40203
0.40 µm -60 to 315/330 °C 40210
40211
40212