Basic HTML Version
Sunniest C18
Sunniest RP-AQUA
Sunniest C8
◆
Comparison data: Bleeding from column
Column size: 150 x 2.0 mm
Mobile phase:
A) 0.1% acetic acid
B) CH
3
CN
Gradient:
Time: 0min 3min 14.4min 18min 19min
%B: 5% 5% 100% 100% 5%
Flow rate: 0.2 mL/min
Temperature: 40 ºC
Detection: Corona CAD
Sunniest C18
B
社
C18
F
社
C18
Retention time/min
Area: 960,000
Area: 1,150,000
Area: 94,000
0.E+00
5.E+07
1.E+08
2.E+08
2.E+08
3.E+08
3.E+08
4.E+08
4.E+08
5.E+08
5.E+08
0
5
10
15
20
Time (min)
Intensity (cps)
Column size: 150 x 2.0 mm
Mobile phase:
A) 0.1% acetic acid
B) CH
3
CN
Gradient: Time: 0min 3min 14.4min 18min 19min
%B: 5% 5% 100% 100% 5%
Flow rate: 0.2 mL/min
Temperature: 40 ºC
MS
：
ABI API-4000
Ionization: Turboionspray (cation)
Measurement mode: Q1 Scan m/z 100-1000
B
社
C18
Sunniest C18
《
Comparison using Corona CAD
》
《
Comparison using MS
》
Retention time/min
1
0
2
4
6
8 10 12 14
Sunniest C18
Sunniest RP-AQUA
Sunniest C8
Company E C18
Company F C18
Company G C18
Company H
C18
N=12,200
TF=1.30
N=12,000
TF=1.28
N=12,100
TF=1.21
N=8,500
TF=2.6
N=5,100
TF=2.4
N=8,100 TF=2.0
N=5,500 TF=2.1
Column size: 150 x 4.6 mm
Particle size: 5
μ
m
Mobile phase: CH
3
CN/10mM
Ammonium acetate pH6.8=40/60
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: Amitriptyline
N
CH
3
CH
3
◆
Separation of antidepressants using acetonitrile and ammonium acetate for LC/MS
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