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Sunniest C18
Sunniest RP-AQUA
Sunniest C8
◆
Relationship between pH and retention of acidic, basic and neutral compounds
pH
Retention factor (k)
Neutral compound (N)
Basic compound (B)
Acidic compound (A)
pH7
pH2
pH10
B
B
B
N
N
N
A
A
A
0
5
10
15
20
25
30
Retention time/min
1
1
2
2
3
3
4
4
5
5
6
6
6
1
4
2
3,5
Column: Sunniest C18, 5
μ
m 150 x 4.6 mm
Mobile phase:
A1) 20mM Phosphoric acid pH2.3
A2) 20mM Phosphate buffer pH7
A3) 20mM Phosphate buffer pH10
B) Acetonitrile
Time (min) 0 30
%B (%) 2 26
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Detection: UV@250 nm
Sample: 1= Thiamine HCl Vitamin B
1
2 = Nicotinamide
3 = Nicotinic acid
4 = Pyridoxine HCl Vitamin B
6
5 = Folic acid
6 = Riboflavin Vitamin B
2
◆
pH selectivity
pH2.3
pH7
pH10
pH of mobile phase can make selectivity of ionic compounds change much. Sunniest
C18 can be used at the pH range from 1.5 to 10, so that a suitable analytical method
can be created using Sunniest C18.
Website : www.chromtech.net.au E-mail : info@chromtech.net.au telNo : 03 9762 2034 . . . in AUSTRALIA