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Acidic pH test
Durable test conditions
Column: Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Mobile phase: 0.5% TFA
Flow rate: 1.0 mL/min
Temperature: 60 ºC
Basic pH test
Sunniest C18
Sunniest RP-AQUA
Sunniest C8
Measurement conditions
Column: Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Mobile phase:
CH
3
OH/H
2
O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil
2 = Amylbenzene
Durable test conditions
Column: Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Mobile phase: 20mM Phosphate buffer pH8.0
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Measurement conditions
Column: Sunniest RP-AQUA, 5
μ
m 150 x 4.6 mm
Mobile phase: 10mM Phosphate buffer pH7.0
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Thymine
Stability of Sunniest RP-AQUA under 100% aqueous conditions
20
30
40
50
60
70
80
90
100
0
20
40
60
80
100 120 140
Time (h)
Relative retetion of ethylbenzene (%)
Sunniest RP-AQUA
It is important that stability is evaluated under 100% aqueous conditions for an AUQA type C18 column because column
life becomes longer to increase a content of organic solvent in a mobile phase. Sunniest RP-AQUA can be used under
100% aqueous conditions from pH2 to pH8.
Sunniest RP-AQUA can be used under 100% aqueous conditions from pH 2 to pH 8. Sunniest RP-
AQUA is one of the most stable aqua type column.
20
30
40
50
60
70
80
90
100
0
100
200
300
400
Time (h)
Relative plate number of thtmine (%)
Sunniest RP-AQUA
Website : www.chromtech.net.au E-mail : info@chromtech.net.au telNo : 03 9762 2034 . . . in AUSTRALIA