R e s t e k
Theoretical Plates andFast LC
•
The longer the column, themore theoretical plates
the column is capable of providing.
•
Shortening columnswill reduce runtime at the
expense of resolution.
•
The loss in resolution can sometimes be
compensated by the use of smaller particles.
•
To achieve the separationwith a shorter column,
the important factor is tomaintain the number of
theoretical plates necessary for the resolution.