R e s t e k
Abstract
Fast LCmethods canbe createdby taking advantage of awide
range of separationvariables tooptimize resolution. Increases in
resolution are created through increases in theoretical plates,
selectivity, and retention. Alpha, the selectivityvariable, is the
greatest factor in separationoptimization. Sometimes a simple
phase change is all that is needed to convert amethod to aFast LC
separation. Optimizationof alpha through stationaryphase
selection can change a gradient system to a faster isocratic system,
allow creationof a singlemethod in lieuofmultiple analysis
methods, and reduce analysis times to a fractionof the original.
This study examines the variables of the resolution equation and
gives significant attention to the optimizationof alpha inHPLC
separations.
