Table II. Experimental conditions for theLC/MS analysis of HMF.
HPLCConditions
Column:
UltraC18 (RestekCorp.), 250mm x 4.6mm, 5 µm
MobilePhaseA: 90:10water:methanol, 10mMammonium formate
MobilePhaseB: 10:90water:methanol, 10mMammonium formate
Gradient:
Sameas inTable I
Inj. Volume:
10µL
FlowRate:
0.3mL/min toUVdetector, 0.2mL/min toMSD
MSDConditions
Detector:
MicromassZMD
Mode:
ESI+
CapillaryV:
3.08V
Extractor V:
13V
IonEnergy:
0.5
Multiplier:
650
SourceTemp:
100°C
Desolv. Temp:
200°C
GasFlow:
500L/hr.
SIM ion:
m/z =127
TIC range:
m/z =80–180
