Table I. Chromatographic conditions for HMFanalysis by LC.
Column: UltraC18 (RestekCorp.), 250mm x 4.6mm, 5µm
MobilePhaseA: 90:10water:methanol, 10mMammonium formate
MobilePhaseB: 10:90water:methanol, 10mMammonium formate
Time (minutes)
%B
0
0
5
0
10
100
20
100
21
0
Flow:
0.5mL/min.
Temperature:
ambient
Detector:
UV@280nm
InjectionVolume: 10µL
