min.2 4
6 8
10 12
14 16
For permission to reproduce any portion of this application note, please contact Restek’s publications/graphics department by phone (ext. 2128) or FAX.
Rtx-5Amine (Fused Silica)
(Crossbond
®
5% diphenyl/95% dimethylpolysiloxane) Stable to 340°C
ID:
df (µm)
temp. limits
15-meter
30-meter
0.25mm
0.50
-60 to 300/315°C
12335
12338
1.00
-60 to 300/315°C
12350
12353
0.32mm
1.00
-60 to 300/315°C
12351
12354
1.50
-60 to290/305°C
12366
12369
0.53mm
1.00
-60 to 290/305°C
12352
12355
3.00
-60 to 280/295°C
12382
12385
©Copyright 2001, RestekCorporation
Figure 2
shows the analysis of BrandAandBrandBmedica-
tions, respectively, using anRtx
®
-5Amine column.
Phenylpropanolamine and guaifenesin arewell resolved from
each other with no apparent interferences from the sample
matrix. Based on the response of phenylpropanolamine inBrand
A, concentrations as low as 10µg/mLcan be detected by adjust-
ing the sample size in the extraction procedure.
Cold and sinusmedications can be checked quickly and easily for
the presence of phenylpropanolamine using the procedure
described above. Complete resolution of all analytes, and opti-
mized peak shape and response can be achieved by selecting the
appropriate column phase and dimensions.
Figure 2:
The analysis of Brand A and Brand Bmedications using anRtx
®
-5Amine column.
min.2 4
6 8
10 12
14 16
1. R. Horwitz, MD, L. Brass, MD, W. Kernan, MD, C. Viscoli, PhD, “Phenylpropanolamine andRisk of Hemorrhagic Stroke: Final Report of TheHemorrhagic Stroke
Project”, May 10, 2000.
1
3
3
Brand A
The Rtx
®
-5Amine column resolves
phenylpropanolamine and
guaifenesinwith no other apparent
interferences from the samplematrix.
Brand B
No phenylpropanolamine is detected
using the Rtx
®
-5Amine columns.
BrandA andBrandB:
Rtx
®
-5AmineColumn
30m, 0.53mm ID, 1.00µm df (cat.# 12355)
Oven temp.:
175°C to 280°C@
10°/min. (hold 5.5min.)
Inj./det. temp.:
250°C/280°C
Carrier gas:
helium
BrandA andBrandB:
Peak List:
1. phenylpropanolamine
2. phenylephrine
3. guaifenesin
4. pheniramine
5. phenyltoloxamine
6. chlorpheniramine
Linear velocity:
40cm/second
Sample size:
1uL
Split vent flow:
88mL/min.
Split ratio:
20:1
7. brompheniramine
8. dextromethorphan
9. pyrilamine
10. codeine
11. hydrocodone
Refer toRestek’s Product Guide for
Base-DeactivatedGuard Columns
and Inlet Liners!
