292-293 / 582
0
2
4
6
8
10
Time (min)
0
2
4
6
8
10
Time (min)
Conventional C18
3
6
1 / 2
5
4
20% Acetonitrile
2
0
2
4
6
8
10
Time (min)
PFP Propyl
5
4
3
6
1
60% Acetonitrile
Cyanopropyl
6
1
5
4
2/3
20% Acetonitrile
Fluoroquinolone Stationary Phase Selection
Mobile Phase – (A)10mM Potassium
Phosphate (pH 2.5): (B) ACN
Flow rate: 1.0 mL/min
UV detection @ 220 nm
Sample Volume: 5µl of 50µg/mL
5um, 150 x 4.6mm
5um, 150 x 4.6mm
5um, 150 x 4.6mm
Selectivity = better MS methods