HPLC COLUMNS
Physical Characteristics of HPLC Columns; USP Descriptions
6
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USPharmacopoeiaCrossReference
pH ranges and temperature limits: see product listings on pages listed here.
Column lifetimewill be shorter when operating at pH and/or temperature extremes.
L1
Octadecyl silane chemically bonded to porous silica or ceramicmicroparticles, 1.7 to 10µm in diameter, or amonolithic rod.
Allure
®
C18 (p. 18), Pinnacle
™
DB C18 (p. 12), Pinnacle
™
II C18 (p. 14), Ultra C18 (p. 22), Allure
®
Aqueous C18 (p. 19), Ultra Aqueous C18 (p. 23), Viva C18 (p. 29),
Kromasil
®
C18 (p. 32)
L3
Porous silica particles, 5 to 10µm in diameter.
Allure
®
Silica (p. 21), Pinnacle
™
DB Silica (p. 13), Pinnacle
™
II Silica (p. 17), Ultra Silica (p. 27), Viva Silica (p. 31), Kromasil
®
Silica (p. 33)
L7
Octylsilane chemically bonded to totally porous silica particles, 1.7 to 10µm in diameter.
Pinnacle
™
DB C8 (p. 12), Pinnacle
™
II C8 (p. 15), Ultra C8 (p. 22), Viva C8 (p. 29), Kromasil
®
C8 (p. 33)
L8
An essentiallymonomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3 to 10µm in diameter.
Pinnacle
™
II Amino (p. 16), Ultra Amino (p. 26), Kromasil
®
Amino (p. 33)
L10
Nitrile groups chemically bonded to porous silica particles, 3 to 10µm in diameter.
Pinnacle
™
DB Cyano (p. 13), Pinnacle
™
II Cyano (p. 15), Ultra Cyano (p. 25), Allure
®
Basix (p. 18)
L11
Phenyl groups chemically bonded to porous silica particles, 1.7 to 10µm in diameter.
Pinnacle
™
DB Phenyl (p. 13), Pinnacle
™
II Phenyl (p. 16), Ultra Phenyl (p. 25), Allure
®
Biphenyl (p. 20), Pinnacle
™
II Biphenyl (p. 17), Viva Biphenyl (p. 30),
Kromasil
®
Phenyl (p. 33)
L13
Trimethylsilane chemically bonded to porous silica particles, 3 to 10µm in diameter.
Ultra C1 (p. 24), Kromasil
®
C1 (p. 33)
L26
Butyl silane chemically bonded to totally porous silica particles, 3 to 10µm in diameter.
Ultra C4 (p.24), Viva C4 (p.30), Kromasil
®
C4 (p.32)
L43
Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to 10µm in diameter.
Allure
®
PFP Propyl (p. 19), Ultra PFP (p. 26), Viva PFP Propyl (p. 30)
Applications
Carbon
load (%)
Pore
Size (Å)
End
Cap?
Restek
HPLCColumn
Ultra PFP
Y
100
7 Ideal for taxol and precursors, or halogenated compounds, amines, esters, or ketones.
Ultra Silica
NA
100
NA Ideal for normal phase applications.
Ultra Carbamate
100
Rapid analysis of carbamates.
UltraQuat
— 100
— Proprietary phase for the analysis of paraquat and diquat and other quaternary amines.
Kromasil
®
C18
Y
100
20
Kromasil
®
C8
Y
100
12
Kromasil
®
C4
Y
100
8
Kromasil
®
C1
Y
100
—
Kromasil
®
Phenyl
Y
100
14
Kromasil
®
Amino
Y
100
1.7%
nitrogen
Kromasil
®
Silica
— 100
—
VivaWide Pore C18
Y
300
9 Proteins and other highermolecular weight compounds.
VivaWide Pore C8
Y
300
5 Proteins and other highermolecular weight compounds. Less retentive than C18 phase.
VivaWide Pore C4
Y
300
3.5 Proteins and other highermolecular weight compounds. Less retentive than C18 and C8 phases.
VivaWide Pore
Biphenyl
Y
300
6.7 Exhibits excellent peak shape for awide range of compounds; ideal for largemolecule and biomolecule assays.
VivaWide Pore
PFPPropyl
Y
300
5
Exhibits excellent peak shape for awide range of compounds, including nucleosides, nucleotides,
and halogenated compounds.
VivaWide Pore Silica — 300
— Normal phase applications for highly retained highmolecular weight compounds.
pHidelity
®
C18
NA
200
—
Hydrophobic C18 phase suitable for analyzing awide range of compounds;
enhanced stability under basic and acidic conditions.
