HPLC COLUMNS
Physical Characteristics of Restek HPLC Columns
5
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Chromatographic Properties
Similar Phases
USP
Code Page#
Highly base-deactivated spherical silicamanufactured by Restek. Monomeric C18 bonding.
Hypersil
®
BDS C18, Zorbax
®
Eclipse XDB-C18 L1
12
Highly base-deactivated spherical silicamanufactured by Restek. Monomeric C8 bonding.
Similar to Pinnacle
™
DB C18, but the shorter alkyl chain provides less hydrophobic retention.
Hypersil
®
BDS C8
L7
12
Highly base-deactivated spherical silicamanufactured by Restek. Cyano bonding.
Hypersil
®
BDS Cyano,
Zorbax
®
Eclipse XDB-CN
L10
13
Highly base-deactivated spherical silicamanufactured by Restek. Phenyl bonding.
Hypersil
®
BDS Phenyl,
Zorbax
®
Eclipse XDB-Phenyl
L11
13
Highly base-deactivated spherical silicamanufactured by Restek.
—
L3
13
Intermediate carbon load and surface area, suitable for awide range of neutral to acidic compounds.
Silicamanufactured by Restek.
Hypersil
®
ODS, Spherisorb
®
ODS
L1
14
Proprietary stationary phase; resolves 16 PAHs inUS EPAMethod 610. Silicamanufactured by Restek.
Unique
— 14
Provides shorter retention times for hydrophobic compounds than C18. Silicamanufactured by Restek.
Hypersil
®
C8, Spherisorb
®
C8
L7
15
More rugged than bare silica for normal phase analyses. Silicamanufactured by Restek.
Hypersil
®
CPS, Spherisorb
®
Cyano
L10
15
Offers unique selectivity versus traditional alkyl chain phases, especially for aromatic compounds.
Silicamanufactured by Restek.
Hypersil
®
Phenyl, Spherisorb
®
Phenyl
L11
16
Silicamanufactured by Restek.
Hypersil
®
APS 2 Amino, Spherisorb
®
Amino L8
16
Silicamanufactured by Restek. Unique biphenyl phase.
Unique
L11
17
Superior value phase for normal phase separation of polar analytes. Lower retention thanUltra C18.
Silicamanufactured by Restek.
Hypersil
®
Silica
L3
17
Most retentive phase for hydrophobic and slightly polar analytes. Mobile phase containing higher percentage
of organicmodifier contributes to higher sensitivity in ESI-based LC/MS.
Ultracarb
®
C18, BetaMax
®
Neutral,
Discovery
®
C18
L1
18
Highly retentive and selective for reversed phase separations of polar analytes. Highly base deactivated.
Compatiblewith highly aqueous (up to 100%) mobile phases.
—
L1
19
Highly retentive, highly selective phase, developed specifically for the analysis of aldehydes and ketones
as DNPH derivatives.
Unique
— 21
Highly retentive phase for analytes containing amino functionality.
BetaMax
®
Base, Maxsil
™
CN
L10
18
A pentafluorophenyl phasewith a propyl spacer. Highly retentive for basic analytes. Excellent for beta-blockers,
halogenated compounds, nucleosides, nucleotides, pyridines, pyrimidines, tricyclic antidepressants.
Discovery
®
HS F5
— 19
Single 30cm column performs equally to two C18 columns in series. (AOACMethod 986.13)
Unique
— 20
High purity, highly retentive phase for aromatic and unsaturated compounds.
Unique
L11
20
High purity, highly retentive phase for normal phase separation of polar analytes. Very high surface area.
Maxsil
™
Si
L3
21
A very retentive, high-purity phase that exhibits excellent peak shape for awide range of compounds.
Recommended as a general purpose reversed phase column.
Discovery
®
C18, Symmetry
®
C18,
Hypersil
®
Gold C18, Luna
®
C18, Zorbax
®
C18,
Kromasil
®
C18, LiChrospher
®
RP-18,
Inertsil
®
ODS-2, Develosil
®
C18
L1
22
Highly retentive and selective for reversed phase separations of polar analytes. Highly base deactivated.
Compatiblewith highly aqueous (up to 100%) mobile phases.
AQUA
®
C18, Aquasil C18, Hypersil
®
Gold AQ,
YMC
®
ODS-Aq
L1
23
One of a group of intrinsically base-deactivated (IBD) phases, with a polar groupwithin, or intrinsic to,
the alkyl bonded phase. Provides unique selectivity and high level of base deactivationwhile reducing or
eliminating the need formobile phase additives.
SymmetryShield
™
, Discovery
®
ABZ&ABZ+,
Prism
™
— 22
Very retentive, high-purity, base-deactivated reversed phase packing that exhibits excellent peak shape
for awide range of compounds.
Luna
®
C8, Symmetry
®
C8, Hypersil
®
Gold C8 L7
23
Exceptionally stable C4 packing, with high bonding coverage and silanol base-deactivation.
Exhibits shorter retention than C18 or C8 phases.
Supelcosil
™
Butyl (C4), Delta-Pak
™
C4
L26
24
Exceptionally stable C1 packing resists hydrolysis, even under acidicmobile phase conditions.
Least retentive reversed phase hydrocarbon packing.
Spherisorb
®
C1
L13
24
High-purity cyano phasewith reduced silanol activity. Often a better choice than C18 for basic pharmaceuticals.
Cyano is themost stable bonded phase for normal phasemode.
Platinum
™
CN, Develosil
®
Cyano, Luna
®
CN,
Hypersil
®
Gold CN
L10
25
High-purity, highly retentive, base-deactivated phasewith alternate selectivity to hydrocarbon phases,
especially for aromatic analytes.
Platinum
™
Phenyl, Supelcosil
™
Phenyl,
Betasil
®
Phenyl
L11
25
Recommended for normal phase analyses of mono- and disaccharides and other similar compounds.
Can also serve as aweak anion exchanger, with aqueous buffers.
Platinum
™
Amino, Develosil
®
NH2
L8
26