Figure2
Stationaryphasecomparison.
HPLC COLUMNS
Column Selection
3
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Cyano&Fluorinatedphases
Fluorinatedphases (such as the
pentafluorophenyl propyl (PFP
propyl)) and cyano-basedphases
interact stronglywithbasic, nitro-
gen-containing andhalogenated
analytes.
Phenyl&Biphenylphases
Phenyl stationary phases interact
with compounds containing aro-
matic groups or unsaturatedbonds
through
π
-
π
interactions. The
biphenyl stationary phase has even
greater interactiondue to the high-
er concentrationof aromatic rings.
Alkylphasewithpolar
functionalgroup
An alkyl-based stationary phase
with either an embeddedpolar
group (P1), a polar side chain (P2),
or a polar end-cap (P3), has signifi-
cantly greater interactionwith
polar compounds than a tradition-
al alkyl phase.
Alkylphases (e.g.C18)
Alkyl-based stationary phases, such
asC18, are best suited for analyzing
hydrophobicmoleculeswith a high
carbon:heteroatom ratio.
CH
3
Side
CH
2
C18
PolarGroup
Conventional
Propyl Phenyl
Pentafluorophenyl
Propyl
SilicaSurface
SilicaSurface
SilicaSurface
SilicaSurface
Cyano
Biphenyl
Si
Si
Si
Si
Si
Si
P1
P2
P3
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
F
F
F
F
F
Si
Si
Si
Si
C
N
Si
Si
Si
Si
Si
Si
