General Operating Procedure
LargeVolumeLiquid InjectionSteps
4. Inject1µL to200µLof liquidsample into thePTV injector. In the“ExpectedPerformance”example,
100µLofC10-C28hydrocarbonmixturewas injected.
2. Type inaneventprogramasfollows:
Time Event
0.00 ZERO
3.00 AOFF(splitventclosed; ifyouget too largeasolventpeak,keep thesplitventopen
longer)
3.10 BON (carrierOFF)
3.20 CON (PTV injectorheat)
5.00 BOFF (carrierON)
INJECTORS
PTV -ProgrammedTemperatureVaporization Injector
1. Thesplitventmustbeopenedmanuallyprior to the runbyactivatingoneof the relayoutputs from the
PeakSimpledatasystem. RelayAistypicallyusedtoactivatethesplitventsolenoid. Ifanotherrelayhasbeen
allocatedtothisfunction,itwillbenotedintherelayassignmentchartlocatedontherighthandsidepanelofthe
GC. Enter thedesiredrelaycommands in thePeakSimpleEvents table. Thesplitventcanalsobe turnedON
(orOFF)byopening therelaywindow thenclickingon the letterA.
5. Hit theRUNbuttononyourGCorpress thespacebaronyourcomputerkeyboard.
Open the Relay window in PeakSimple
byclickingon theViewdrop-downmenu
and choosingRelay/pumpwindow
Example PeakSimple PTV event table
3. CarriergasexitsthesplitventonlywhenRelayAisactivated. Connectaflowmeasuringdevicetothesplit
ventexit tube. Lower theGC lid(whenopen, lid interlockdisables thesolenoidfunction),activateRelayA,
andadjust theneedlevalve to thedesiredflow. Formost liquid injectionsusingaPTV, thesplitventshouldbe
wideopen. Thisallows the trappingmaterial toretain thecompoundsof interestandquicklyflush thesolvent
tovent. If thesplitratio isset too low,someof thesolventandanalytesmayenter thecolumnbefore thePTV
injector isheatedup, resulting insmearedordoublepeaks.
