Chapter. Injectors
Topic:
On-column InjectorOperation
5) Tignten the septum nut unt! rtcontacts
tile one
Of
two
rubber
o-rings
OIl
me injec–
tor
bo<:l1
The
o-nngs
act
as
I
nelpful
gYldI! to aVOId over-bgnlen"lg
l/Ie
septum
When the
soft
silicone
ru~
of
ttle
sep–
tum
is
over-compressed,
ee
syrinlle has
to
figllt
lIS
way througn
oft...,
plugging ",,",n
septum malerial in
l/Ie
process
A
prop–
erl\tbgnlened septum
cll!a.es
easily
to
let
l/II!
s~nge
needle
pass.
ttIe<1
se"·l>eals
rtse" wlIen
ee
syringe
is
wllhdrawn.
Properl\tbghtened. a plug
t)'pe
seplym
as
Ysed
on
lhe
SRIGC
wil last
"Il
to 300
InjedJons,while an
OVI!f-~ght""ed
Ol'll!
will
leak
aller
10·20
injl!Cbons.
Tn" pnotoabove shows the inJl!<;to< filling
with
l'Ml
o-rings installed
OIl
rt. and the
51!\11um nut
tightened
up
50
rt just
con–
tacts
l/II!
o-rings
If
the
synnge snags on ltIe
edge
of
!he
cojumn
es
~
is
inserted, loosen
the
swagelok nut and ferrule and
pull
the
c0l–
umnaoolher
lew
millimeters
turthe<
to–
wards the ,nSlOe
of
tt>e
cojumn
0'1""
Tightan
me
nut and
retest
tljI
inserting
!he
synnge
M.., l t ~.o"h
Test your " s\allahon
by
Insertinga s1nnge into the
~umn
as far as
~
WIll
go_
The synnge should glide
,nto
the
cojumn bore
smoothly
WithOut Sl\8gging
or
I~ing
rougl1
