Chapter: Injectors
4) Pull
me
columnand wide-bor.. adapter
back
into
me
injeclor
fitting
through
Il>e
partia lly
lightened nut and graphite
fer_
rule
As
you
pull,
eecolumn
wi ll
gradu–
~Iy
disappear fromview inside
Il>e
inJec–
tor
litIing.
Pull
the
column unto
trle
open
end
IS
abolJt
halfway
into the
I'ittJng
The
exact
distance
IS
no!
cnhcal !SO
long
as
trle
svnngeneedle ellds up deposlling
the
liquid sample in the bore of
the
column
itself. lIthe
column is pulled
too
lar
to>
wards
the
oven,
Il>e
syringe
needle
may
de\lOSIlthe
sample
In
the
adapter _ re
it
WIllgradually diffuse
into the column
caUslng wide or tailing peaks II the
c0l–
umn
is
pos;boned
too
faroul
towards the
operator,
the
symge
needle
may
_g
on
the
lip
01
the column
as
it
is insefted
W~h
the
column
pos;boned,
lighten the
nut
andgraphite !elTUle.
You
should
fee!
the !emile
squis/1 slightly as you tighten
the lerrule.
and
the
column should
!eel
snugand immovable. A property tight–
ened
ferrule
can
be re-used
5-10
times,
while a ferrule
which
i$
OYer--llghtenecl
must
be
repIace<l
eve<y
bme
Il>e
column
is changed
sors
Metal
cafNllary
cclmrms
aro
rJ8SI8f
10
in–
mil
fIlan
poIyJmida
coated
fused
silica
columns
because
as
the
syringeenters
mecolumnentrance
iI
can
chip
awaybits
of
lho
fused
si/i';a
unJass
ilLs
pertec~y
pi;
siIioned
TIl6
metal
columns
aremore
IofpMng
SlfrCft
me
column
will
nol
fnK:!urfI
when
in
contact
willi
me syringe
ntJedIe
A
7/16"
wrench
IS
used
to
snug up
the ......
alld grapt>–
ite ferrule secunng the column
to
the In,ectof
