42702
-60 to 330/350°C
0.18
0.18mm
temp. limits
df (µm)
ID
• 3 •
800-356-1688
w/ data pack
w/data pack
Rtx®-5SilMSColumns (fusedsilica)
(Selectivity equivalent to Crossbond
®
5% diphenyl / 95% dimethyl polysiloxane)
8270MegaMix™
(76 components)
1,000µg/mL each (3-methylphenol and 4-methylphenol at
500µg/mL each) inmethylene chloride:benzene (75:25),
1mL/ampul
Each
5-pk.
10-pk.
31686
31686-510
—
w/data pack
31686-500
31686-520
31786
acenaphthene-d10
chrysene-d12
1,4-dichlorobenzene-d4
naphthalene-d8
perylene-d12
phenanthrene-d10
SV InternalStandardMix
Each
5-pk.
10-pk.
2,000µg/mL each inmethylene chloride, 1mL/ampul
31206
31206-510
—
31206-500
31206-520
31306
4,000µg/mL each inmethylene chloride, 1mL/ampul
31006
31006-510
—
31006-500
31006-520
31106
2-fluorobiphenyl
nitrobenzene-d5
p
-terphenyl-d14
B/NSurrogateMix (4/89SOW)
2-fluorophenol
phenol-d6
2,4,6-tribromophenol
AcidSurrogateMix (4/89SOW)
compounds, plus benzoic acid, benzidine, and sur-
rogate and internal standards, on our new 20m,
0.18mm ID, 0.18µmRtx
®
-5Sil MS column (cat.#
42702). The Rtx
®
-5Sil MS stationary phase is based
on a silarylene polymer specifically designed for the
demanding GC/MS analysis of semivolatile com-
pounds, and the column exhibits lower bleed than
columns prepared from phenyl/methyl polymers. All
target compounds can be quantifiedwith greater
sensitivity. The thin phase film in this column allows
superior resolution of structural isomers
benzo(b)fluoranthene and benzo(k)fluoranthene
(peaks 83 and 84), while achieving a very short
analysis time of less than15minutes. Peak shape and
response are excellent, even for active compounds
such as 2,4-dinitrophenol and pentachlorophenol
(peaks 50 and 66). Optimizing the temperature pro-
gram, as well as the physical dimensions of the col-
umn, contributes to better resolution of closely elut-
ing peaks and shortens the analysis time.
In order to achieve the separation shown in Figure
1, caremust be taken to optimize injection condi-
tions. To reduce solvent effects that could interfere
withN-nitrosodimethylamine and pyridine (peaks 1
and 2), we chose a splitless inlet liner, rather than a
direct injection liner (e.g., a Uniliner
®
). A cyclo dou-
ble gooseneck design enables the sample to be com-
pletely volatilized in the injection port prior to con-
densing at the column inlet, and ensuresmore
reproducible results, relative to a standard (straight)
liner. The 2mm internal diameter provides the best
results with 0.5µL injections. The splitless hold time
also is very important: a change of only several sec-
onds can reduce sensitivity by 50%. We discovered
that a pulsed splitless analysis, using a 0.20min.
pulse 5psi higher than the column flow backpres-
sure, dramatically improves sample transfer onto the
column. Making the pulse 3 seconds (0.05min.)
longer than the splitless hold time (0.15min.)
allows excess solvent to be swept away quickly. The
270ºC injection port temperature vaporizes the
Each
5-pk.
10-pk.
1,000µg/mL each inmethylene chloride, 1mL/ampul
31024
31024-510
—
w/ data pack
31024-500
31024-520
31124
5,000µg/mL each inmethylene chloride, 1mL/ampul
31062
31062-510
—
w/ data pack
31062-500
31062-520
31162
5,000µg/mL each inmethylene chloride, 5mL/ampul
31086
31086-510
—
w/ data pack
31086-500
31086-520
31186
w/ data pack
Each
5-pk.
10-pk.
2,000µg/mL each inmethanol, 1mL/ampul
31025
31025-510
—
w/ data pack
31025-500
31025-520
31125
10,000µg/mL each inmethanol, 1mL/ampul
31063
31063-510
—
w/ data pack
31063-500
31063-520
31163
10,000µg/mL each inmethanol, 5mL/ampul
31087
31087-510
—
31087-500
31087-520
31187
samplewithminimal analyte breakdown. GC condi-
tions were adjusted to resolve analytes that coelute
and share ions. Aniline and phenol (peaks 4 and 6),
for example, were resolved by using an initial tem-
perature ramp rate of 14ºC/min., and the key to
resolving isomers benzo(b)fluoranthene and
benzo(k)fluoranthene (peaks 83 and 84) is to be
sure that they elute during the temperature ramp
portion of the program. If the isomers elute during
the final hold time they will not bewell resolved. By
using a 0.18mm IDRtx
®
-5Sil MS column under
these conditions, youwill ensure a rapid and suc-
cessful analysis of the 8270 compounds.
Tomeet the substantial demand for referencemate-
rials forMethod 8270, we offer 8270MegaMix
™
ref-
erencemix (cat.# 31686)—a formulation of 76 tar-
get compounds inmethylene chloride/benzene
(75:25).
continued on page 5
ID
df (µm)
temp. limits
15-Meter
30-Meter
0.25mm
0.25
-60 to 330/350°C
12720
12723
0.50
-60 to 330/350°C
12735
12738
1.00
-60 to 325/350°C
12750
12753
0.28mm
0.25
-60 to 330/350°C
12790
12793
0.50
-60 to 330/350°C
12791
12794
1.00
-60 to 325/350°C
12792
12795
0.32mm
0.25
-60 to 330/350°C
12721
12724
0.50
-60 to 330/350°C
12736
12739
1.00
-60 to 325/350°C
12751
12754
Components listed in
bold
in Figure 1.
Benzidine
1,000µg/mL inmethanol, 1mL/ampul
Each
5-pk.
10-pk.
31441
31441-510
—
w/data pack
31441-500
31441-520
31541
BenzoicAcid
1,000µg/mL inmethanol, 1mL/ampul
Each
5-pk.
10-pk.
31415
31415-510
—
w/data pack
31415-500
31415-520
31515
2,4-Dinitrophenol
1,000µg/mL inmethanol, 1mL/ampul
Each
5-pk.
10-pk.
31291
31291-510
—
w/data pack
31291-500
31291-520
31391
Rtx
®
-5Sil MS 20m, 0.18mm ID, 0.18µm (cat.# 42702)
Sample:
US EPAMethod 8270Dmix: 8270MegaMix
™
(cat.# 31686), benzoic acid (cat.# 31415), benzidine (cat.# 31441),
2,4-dinitrophenol (cat.# 31291)*, Acid SurrogateMix (4/89 SOW) (cat.# 31063),
B/NSurrogateMix (4/89 SOW) (cat.# 31062), SV Internal StandardMix (cat.# 31206)
*2,4-dinitrophenol also is a component of theMegaMix
™
.
Inj.:
0.5µL, 5ppm each component (2.5ng on column) (2,4-dinitrophenol at 10ppm/5ng on column; 3-methylphenol and
4-methylphenol at 2.5ppm/1.25ng on column), splitless (hold 0.15min., pressure pulse 0.20min. @ 30psi),
2mm cyclo double gooseneck inlet liner (cat.# 20907); Agilent 6890
Inj. temp.:
270°C
Carrier gas:
helium, constant flow
Flow rate:
1.2mL/min.
Oven temp.:
40°C (hold 0.5min.) to 90°C@ 14°C/min., to 330°C@ 22°C/min. (hold 1min.)
Det.:
Agilent 5973GC/MS
Transfer line
temp.:
280°C
Scan range:
35-550 amu
Solvent Delay:
1min.
Tune:
DFTPP
Ionization:
EI
20-Meter
Use thisnew column for
sub-15minuteanalysisof
78Method8270D target
compounds.
