• 12 •
800-356-1688
Restek HPLCColumn Kits for Faster,
EasierMethodDevelopment
by RebeccaWittrig, Ph.D., HPLCProduct MarketingManager
✔
Multiple stationary phases, for quick optimization of selectivity.
✔
Specific kits forMS or UV detection—both include columns specifically for basic analytes.
✔
Fast LC kits use economical cartridge design.
When developing a newHPLC assay, method devel-
opment chemists often start with a C18 or C8 station-
ary phase, because these phases have proven useful
for analyzing awide range of organic compounds.
Many analysts have learned, however, that a C18 or
C8 stationary phase is not the best choice for every
separation. A cyano-, pentafluorophenyl, or amino-
containing stationary phase, a phasewith embedded
polar groups, or a phase designed for compatibility
with highly aqueousmobile phasesmight provide
superior resolution of target compounds (Figure 1).
To help analysts efficiently select the optimum sta-
tionary phase, we have assembled kits of columns
for use inHPLCmethod development. The four
columns in each kit incorporate a range of station-
ary phase types, are configured for rapid analyses,
and are optimized for the detector type: 50 x 4.6mm
ID columns containing 5µm packings for usewith
UV detection, 30 x 2.1mm ID columns containing
3µm packings for LC/MS. The Fast LCmethod devel-
opment kits include four 30 x 2.1mm ID or four 30
x 4.0mm ID cartridges containing 3µm packings,
and a cartridge holder.
Description
qty.
cat.# price
LC/MS (1 Allure
™
C18 (USP L1), 1 Allure
™
PFP Propyl,
1 Allure
™
Basix (USP L10), 1Ultra Aqueous C18 (USP L1))
four 30 x 2.1mm, 3µm columns 25136
LC/UV (1 Pinnacle
™
DB C18 (USP L1), 1 Allure
™
PFP Propyl,
1Ultra Aqueous C18 (USP L1), 1Ultra IBD)
four 50 x 4.6mm, 5µm columns 25137
FastLCMethodDevelopmentKit
Each kit includes four Fast LC Cartridges (Ultra C18 (USP L1), Ultra Aqueous C18 (USP L1), Ultra Cyano (USP
L10), Ultra PFP (USP L43)) and a Fast LC cartridge holder.
Description
qty.
cat.# price
Fast LCDevelopment Kit
four 30 x 2.1mm columns
25296
Fast LCDevelopment Kit
four 30 x 4.0mm columns
25297
HighPerformance
SilicaProducts
(lit. cat.# 59901)
Tight production
specifications ensure
highly consistent lot-to-lot
performance fromRestek's
proprietary silicamateri-
als.This 6-page publication
characterizes Pinnacle II
™
and Pinnacle
™
DB stock
bulk products—bare silica and silicawith popular
bonded phases—and illustrates typical results
achieved by using them. Custommaterials can be
designed from a broad range of particle sizes, pore
sizes, and phases.
HPLCMethod
DevelopmentCourse
Many analysts anticipate developing a newHPLC
methodwith apprehension.To help de-mystify this
process, Restek, in cooperationwith ChromVision,
offers a dynamic, thorough two-day course on the sub-
ject, presented in a logical and systematicmanner by
an expert on the effects of adsorbent chemistry and
structure onHPLC retention. It provides the knowledge
and tools necessary for understandingwhy a particular
stationary phasewould be chosen for separating vari-
ous analytes, and how to improve selectivity by choos-
ing the proper eluent. Eight lectures cover all aspects
of method development and are complemented and
reinforced by four workshops.The course is especially
useful to analystsworkingwith pharmaceutical or bio-
logical/biochemical analytes. Formore information
about this and other Restek seminars, visit our semi-
narsweb page:
Bulk Silica Available
!
Figure1
Better chromatography for basicmolecules, usinganUltra IBD column.
Thebase-deactivated columnhas auniqueblendof hydrophobic andpolar character for better
resolutionof closely related compounds.
1
2
3
Peak List:
1. uracil
2. famotidine
3. ranitidine
Ultra IBDPhase
•High Selectivity
•Marked Resolution
1
Columns:
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
100Å
Conditions:
Mobile Phase: 20mM K
x
H
y
PO
4
pH 7:acetonitrile (70:30,v/v)
FlowRate:
1.0mL/min
Det.:
UV@234nm
2, 3
ConventionalC18Phase
•Little Retention
•No Resolution
HPLCandLC/MSColumnKits
2
4
6
8
10
12min.
0
2
4
6
8
10
12
14min.
0
If the columns in the kits listed below don’t appear
tomeet your needs, please contact our Technical
Service group or your Restek distributor for infor-
mation about custom kits.
