• 5 •
2003
Volume 3
800-356-1688
Allure
™
OrganicAcids Column (5µm silica; 4.6mm ID)
Peak List:
Ret. Time (min.)
1.tartaric acid
8.03
2.malic acid
11.9
U. unidentified juice component
Sample:
grape juice
Inj.:
10µL
Conc.:
grape juice:water (50:50, v/v)
Sample Diluent:
purifiedwater
Column:
Allure
™
Organic Acids
Catalog #:
9165585
Dimensions:
300 x 4.6mm
Particle Size:
5µm
Pore Size:
60Å
Conditions:
Mobile Phase:
100mM phosphate buffer, pH 2.5
Flow:
0.5mL/min
Temp.:
ambient
Det.:
UV@ 226nm
Figure 2
– Sharp, easily differentiated organic acid profiles for
grape juice and cranberry juice cocktail, provided by an Allure
™
Organic Acids column.
LC_0237
Peak List:
Ret. Time (min.)
1.quinic acid
8.88
2.malic acid
11.92
3.citric acid
27.00
U. unidentified juice component
Sample:
cranberry juice cocktail
Inj.:
10µL
Conc.:
cranberry juice cocktail:water (50:50, v/v)
Sample Diluent:
purifiedwater
LC_0236
BaselineSeparationof
PAHsbyHPLC,Usinga
Pinnacle
II
™
PAHColumn
✔
Fast analysis— resolve 16 target PAHs in less than 18
minutes!
✔
Excellent resolution— cross-linkedC18 phase gives
baseline separation.
✔
Column-to-column reproducibility—we control the
entiremanufacturing process: silica production -
columnmanufacture - final testing. Youwill see the same
outstanding performance from every Pinnacle
II
™
PAH
column you use!
6
8
10
12
14
16
18
1
2
3
4
6
7 8
9
11
13
12
15
16
14
10
5
Column:
Pinnacle
II
™
PAH
Catalog #:
9219563
Dimensions:
150 x 3.2mm
Particle Size:
5µm
Pore Size:
110Å
Conditions:
Mobile Phase: A: purifiedwater, B: acetonitrile
Time (min.)
%B
0
40
7
60
11
100
17.9
100
18
40
Flow:
1.2mL/min
Temp.:
ambient
Det.:
UV@ 254nm
Sample:
Inj.:
5µL PAH standard
(cat.#31264)
Conc.:
see peak list
Sample Diluent:
1:9methylene
chloride:acetonitrile
Sample Temp.:
ambient
LC_0303
Figure 1 -
Baseline separation of US EPAMethod 610 PAHs
in <18minutes.
Peak List:
Conc. (µg/mL)
1. naphthalene
100
2. acenaphthylene
100
3. acenaphthene
100
4. fluorene
100
5. phenanthrene
50
6. anthracene
100
7. fluoranthene
50
8. pyrene
50
9. benzo(a)anthracene
50
10. chrysene
50
11. benzo(b)fluoranthene
50
12. benzo(k)fluoranthene
50
13. benzo(a)pyrene
50
14. dibenzo(a,h)anthracene 50
15. benzo(ghi)perylene
50
16. indeno(1,2,3-cd)pyrene 50
Other referencemixes available—
please see our catalog or website.
An example chromatogram fromour new
Environmental HPLC
flyer.
Request your copy today! lit. cat.#59741
Grape Juice
Cranberry
Juice Cocktail
citric acid
2000µg/mL
fumaric acid
10
malic acid
2000
quinic acid
2000
tartaric acid
2000
OrganicAcids ReferenceMixture
min.
min.
Baseline resolution
in less than
18minutes!
U
U
Length
cat.#
150mm
9165565
250mm
9165575
300mm
9165585
Each
5-pk.
10-pk.
Inwater, 1mL/ampul
35080
35080-510
—
35080-500*
35080-520*
35180*
Inwater, 5mL/ampul
35081
35081-510
—
35081-500*
35081-520*
35181*
*w/data pack
min.
