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RESTEKAdvantage
800-356-1688
Analysis of Complex
Semivolatiles Samples
Quantify 126SemivolatileCompounds,
Using anRtx
®
-5SilMSCapillaryGCColumn
✔
Full complement of 126 EPA 8270 semivolatile/Appendix IX compounds in 3mixes
✔
New 8270/Appendix IXKit includes all 3mixes
✔
New 32 component Appendix IXMix
By Gary Stidsen, Innovations TeamManager and KatiaMay, Ph.D., R&DChemist
Appendix IX is a list of organic and inorganic
Hazardous Constituentsmonitored in groundwater
during compliancemonitoring and corrective actions
at RCRA-regulated hazardous waste treatment, stor-
age, and disposal facilities. The organics usually are
evaluated by following US EPAMethod 8260
(volatiles), Method 8270 (semivolatiles), orMethod
8080 (organochlorine pesticides).
Restek chemists determined themost commonly
analyzed Appendix IX compounds, carefully reviewed
the latest version of EPAMethod 8270, and designed
a new referencemix of 32 compounds—Appendix
IXMix #2—tomeet current needs of environmental
laboratories.
We formulated the newmixwith the goal of prepar-
ing a product that is stable as well as useful.
Appendix IX constituents includemany classes of
chemicals: polynuclear aromatic hydrocarbons
(PAHs), phenols, chlorinated aromatic hydrocar-
bons, aldehydes, anilines, benzidines, insecticides.
Unstable combinations of these compounds will pro-
duce chemical interactions—and flawed calibration
data. Methylene chloride is a common solvent for
semivolatile organics, but some grades of methylene
chloride contain low concentrations of methanol as a
stabilizer. Aldehydes (e.g., benzaldehyde) and chlori-
nated triazines (e.g., atrazine) can react with
methanol; chlorinated triazines also react withwater.
Consequently, we use affirmedmethanol-free, water-
freemethylene chloride in formulating ourmixes.
We package the newmix in deactivated amber glass
ampuls, to prevent reactions catalyzed by light.
Appendix IXMix #2 is a highly useful complement to
our 8270MegaMix
™
(76 compounds, cat.# 31686)
Ordering Information
|
Rtx
®
-5Sil MS column
30m,
0.25mm ID,
0.25µm
cat.# 12723
and Appendix IXMix #1 (18 compounds, cat.#
31625). The 126 semivolatiles in these threemixes
are separated on anRtx
®
-5Sil MS column (cat.#
12723) (Figure 1, page 15). Because the PAHs elute
at temperatures over 300ºC, the analysis requires a
column that will not bleed at high temperatures.
Further, an inert column is important for the active
compounds (e.g., pentachlorophenol, dinitrophe-
nols). Rtx
®
-5Sil MS columns arewidely used for ana-
lyzing various classes of semivolatiles, because they
exhibit versatility, inertness, and low bleed at high
temperature. The optimal combination of internal
diameter and film thickness of a 30m, 0.25mm ID,
0.25µmRtx
®
-5Sil MS columnmake this the best
choice for analyzing complexmixtures of semi-
volatiles. The Rtx
®
-5Sil MS column, in combination
with our 8270MegaMix
™
mix, Appendix IXMix #1,
and new Appendix IXMix #2, and our internal stan-
dards and surrogates for EPAMethod 8270 and
Appendix IX, make Restek the only place you need to
lookwhen youwant columns and referencemateri-
als for semivolatiles analysis.
Appendix IXMix #2
(32 components)
*Concentration is 500µg/mL.
1
1,2-diphenylhydrazine (8270-listed analyte) decomposes to
azobenzene (mix component).
2
N-nitrosodiphenylamine (8270-listed analyte) decomposes to
diphenylamine (mix component).
acenaphthene
acenaphthylene
aniline
anthracene
azobenzene
1
benzo(a)anthracene
benzo(a)pyrene
benzo(b)fluoroanthene
benzo(ghi)perylene
benzo(k)fluoroanthene
benzyl alcohol
benzyl butyl phthalate
bis 2-ethylhexyl adipate
bis(2-chloroethoxy)methane
bis(2-chloroethyl)ether
bis(2-chloroisopropyl)ether
bis(2-ethylhexyl)phthalate
4-bromophenyl phenyl ether
carbazole
4-chloroaniline
4-chloro-3-methylphenol
2-chloronaphthalene
2-chlorophenol
4-chlorophenyl phenyl ether
chrysene
dibenz(a,h)anthracene
dibenzofuran
1,2-dichlorobenzene
1,3-dichlorobenzene
1,4-dichlorobenzene
2,4-dichlorophenol
diethyl phthalate
dimethyl phthalate
2,4-dimethylphenol
1,2-dinitrobenzene
1,3-dinitrobenzene
1,4-dinitrobenzene
4,6-dinitro-2-methylphenol
2,4-dinitrophenol
2,4-dinitrotoluene
2,6-dinitrotoluene
di-
n
-butyl phthalate
di-
n
-octyl phthalate
diphenylamine
2
fluorene
fluoroanthene
hexachlorobenzene
hexachlorobutadiene
hexachlorocyclopentadiene
hexachloroethane
indeno(1,2,3-cd)pyrene
isophorone
1-methylnaphthalene
2-methylnaphthalene
2-methylphenol
3-methylphenol*
4-methylphenol*
naphthalene
2-nitroaniline
3-nitroaniline
4-nitroaniline
nitrobenzene
2-nitrophenol
4-nitrophenol
N-nitrosodimethylamine
N-nitroso-di-
n
-propylamine
pentachlorophenol
phenanthrene
phenol
pyrene
pyridine
2,3,4,6-tetrachlorophenol
2,3,5,6-tetrachlorophenol
1,2,4-trichlorobenzene
2,4,5-trichlorophenol
2,4,6-trichlorophenol
8270MegaMix
™
(76 components)
1,000µg/mL each (except where noted) in methylene
chloride:benzene (75:25), 1mL/ampul
Each
5-pk.
10-pk.
31686
31686-510
—
w/data pack
31686-500
31686-520
31786
acetophenone
Aramite (2 isomers)
atrazine
benzaldehyde
biphenyl
caprolactam (epsilon)
chlorobenzilate
1-chloronaphthalene
diallate
dibenz(a,h)acridine
2,6-dichlorophenol
7,12-dimethylbenz(a)anthracene
1,4-dioxane
diphenyl ether
ethyl methacrylate
ethyl methanesulfonate
hexachloropropene
isodrin
isosafrole (
cis& trans
)
kepone
methyl methanesulfonate
3-methylcholanthrene
1,4-naphthoquinone
4-nitroquinoline-N-oxide
pentachlorobenzene
pentachloroethane
pentachloronitrobenzene
phenacetin
pronamide
safrole
1,2,4,5-tetrachlorobenzene
1,3,5-trinitrobenzene
1,000µg/mL each inmethylene chloride, 1mL ampul
Each
5-pk.
10-pk.
31806
31806-510
—
w/data pack
31806-500
31806-520
31906
2-acetylaminofluorene
4-aminobiphenyl
p
-dimethylaminoazobenzene
3,3'-dimethylbenzidine
α
,
α
,-dimethylphenethylamine (free base)
methapyrilene (free base)
1-naphthylamine
2-naphthylamine
5-nitro-
o
-toluidine
N-nitrosodibutylamine
N-nitrosodiethylamine
N-nitrosomethylethylamine
N-nitrosomorpholine
N-nitrosopiperidine
N-nitrosopyrrolidine
1,4-phenylenediamine
2-picoline
o
-toluidine
Appendix IXMix #1
(18 components)
2,000µg/mL each inmethylene chloride, 1mL/ampul
Each
5-pk.
10-pk.
31625
31625-510
—
w/data pack
31625-500
31625-520
31725
31686: 8270MegaMix
™
31030: BenzidineMix, EPA 605
31625: Appendix IXMix #1
31806: Appendix IXMix #2
8270/Appendix IXKit
Contains 1mL each of thesemixtures.
Kit
Kit w/Data Pack
31815
31815-500
