At-a-Glance
Product
Information
fromRestek
Rtx
®
-CLPesticidesColumns
faces below thebottomof the liner.Although a splitless injection also canbemade, the
direct injectionmethod provides the best reproducibility, while achieving the required
detection limits andminimizing instrument maintenance.
Themost commonphases used for dual-columnpesticide analysis are cyanopropyl sta-
tionary phases (1701 columns) and phenyl/methyl phases (5, 35, 50 columns). 1701
columns resolve the pesticideswell, but have several limitations: they are prone to on-
columnbreakdownofDDT andmethoxychlor as a result of degradationof the station-
ary phase, and have relatively lowmaximum operating temperatures (275°C).
Variousphenyl/methyl phasesalsocanbeused fordual-columnpesticideanalysis, such
asa5%phenyl/phenylprimarycolumnanda35%ora50%phenyl/methylconfirmational
column.While each of these phases has a highermaximum operating temperature and
is less reactive than the cyano phases, they all have target compounds that coelute to
some extent.
Compound
RetentionTime
(min.)
1. 2,4,5,6-tetrachloro-
m
-xylene
9.34
2.
a
-BHC
10.93
3.
g
-BHC
11.78
4.
b
-BHC
12.02
5.
d
-BHC
12.47
6. heptachlor
13.01
7. aldrin
13.74
8. heptachlor epoxide
15.16
9.
g
-chlordane
15.45
10.
a
-chlordane
15.75
11. 4,4'-DDE
15.92
12. endosulfan I
16.05
13. dieldrin
16.59
14. endrin
17.09
15. 4,4'-DDD
17.22
16. endosulfan II
17.59
17. 4,4'-DDT
17.83
18. endrin aldehyde
18.48
19. methoxychlor
18.87
20. endosulfan sulfate
19.42
21. endrin ketone
20.03
22. decachlorobiphenyl
22.19
On-column concentration:
16-160pg
OrganochlorinePesticideMixAB#2 (cat.#32292)
Ovenprogram:
120°C (hold1min.) to300°C
@9°C/min. (hold10min.)
Inj. port:
Direct injectionusing aUniliner
®
sleeve (cat.#20335) at 200°C
Detector:
ECD at 300°CwithAnodePurge
Dead time:
1.9minutes
Headpressure:
8.7psi (constant)
Flow rate:
He@1.3mL/min.@120°C
Compound
RetentionTime
(min.)
1. 2,4,5,6-tetrachloro-
m
-xylene
9.75
2.
a
-BHC
11.53
3.
g
-BHC
12.48
4.
b
-BHC
12.70
5.
d
-BHC
13.44
6. heptachlor
13.59
7. aldrin
14.37
8. heptachlor epoxide
15.72
9.
g
-chlordane
16.15
10.
a
-chlordane
16.48
11. 4,4'-DDE
16.85
12. endosulfan I
16.61
13. dieldrin
17.22
14. endrin
17.90
15. 4,4'-DDD
18.11
16. endosulfan II
18.36
17. 4,4'-DDT
18.79
18. endrin aldehyde
19.09
19. methoxychlor
20.25
20. endosulfan sulfate
19.69
21. endrin ketone
20.87
22. decachlorobiphenyl
23.84
On-column concentration:
16-160pg
OrganochlorinePesticideMixAB#2 (cat.#32292)
Ovenprogram:
120°C (hold1min.) to300°C
@9°C/min. (hold10min.)
Inj. port:
Direct injectionusing aUniliner
®
sleeve (cat.#20335) at 200°C
Detector:
ECD at 300°CwithAnodePurge
Dead time:
1.9minutes
Headpressure:
8.7psi (constant)
Flow rate:
He@1.3mL/min.@120°C
1
6 7
8 10
11
13
12
14
16
17
18
19
20
21
22
15
2 3
5
4
10
12
14
16
18
20
22
(min.)
9
Figure1:
TheRtx
®
-CLPesticides column (30m, 0.32mm ID, 0.50µm) provides
baseline resolutionof 22 chlorinatedpesticides inEPAMethod8081 in less
than23minutes.
1
6
7
9
8 10
11
13
12
14
16
17
18
19
20
21
22
15
2
3
5
4
Figure2:
TheRtx
®
-CLPesticides2 columnoffers 4 elutionorder changes,
making it the ideal confirmational column.
10
12
14
16
18
20
22
(min.)
24
