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• 2 • 800-356-1688 • 2005 vol. 3
Figure 2 Rapid analysis for volatile anesthetics using
Rtx®-BAC1 and Rtx®-BAC2 columns.
1. isoflurane
2. enflurane
3. halothane
4. methoxyflurane
1.0mL headspace sample
Oven temp.: 40°C (hold 5 min.) to 240°C @ 5°C/min.
Inj. & det. temp.: 240°C
Carrier gas: helium
Linear velocity: 65cm/sec.
GC_PH00501
GC_PH00502
30m, 0.53mm ID, 3.0µm Rtx®-BAC1 (cat.# 18001)
30m, 0.53mm ID, 2.0µm Rtx®-BAC2 (cat.# 18000)
Table I Retention Times for Abused Substances
Rtx ®-BAC1 Rtx ®-BAC2
Elution Ret.Time Elution Ret. Time
Compound Order (min.) Order (min.)
methanol 1 1.017 5 1.237
acetaldehyde 2 1.146 1 1.063
ethyl chloride 3 1.275 2 1.071
ethanol 4 1.299 8 1.648
diethyl ether 5 1.574 4 1.167
isopropanol 6 1.607 15 1.945
isoflurane 7 1.661 13 1.922
methylene chloride 8 1.805 11 1.849
Freon® 113 9 1.864 3 1.145
enflurane 10 1.891 16 2.081
tert-butyl alcohol 11 1.926 17 2.154
acetone 12 1.992 10 1.787
acetonitrile 13 1.997 20 2.553
n-propanol 14 2.191 25 3.130
halothane 15 2.267 18 2.383
methyl tert-butyl ether 16 2.366 7 1.554
hexane 17 2.495 6 1.386
tert-butyl nitrite 18 2.736 9 1.750
chloroform 19 2.870 27 3.290
sec-butyl alcohol 20 2.962 30 3.793
isobutyl nitrite 21 2.973 12 1.853
sec-butyl nitrite 22 3.059 14 1.939
isobutyl alcohol 23 3.460 32 5.100
tetrahydrofuran 24 3.736 24 2.845
methyl ethyl ketone 25 3.768 26 3.271
ethyl acetate 26 3.800 23 2.785
carbon tetrachloride 27 3.842 21 2.565
1,1,1-trichloroethane 28 3.869 22 2.729
n-butyl nitrite 29 3.879 19 2.469
benzene 30 4.186 28 3.392
n-butyl alcohol 31 4.565 33 6.747
trichloroethylene 32 5.205 31 4.084
isoamyl nitrite 33 6.377 29 3.728
methoxyflurane 34 7.279 36 7.219
isoamyl alcohol 35 7.428 38 9.447
toluene 36 8.358 34 6.944
1,1,2-trichloroethane 37 8.498 39 10.138
methyl isobutyl ketone 38 9.510 37 7.964
tetrachloroethylene 39 9.681 35 7.081
chlorobenzene 40 11.810 41 11.012
ethylbenzene 41 12.279 40 10.704
p-xylene 42 12.726 42 11.038
m-xylene 43 12.727 43 11.046
o-xylene 44 13.733 44 12.280
tetrachloroethane 45 14.106 50 16.968
isopropylbenzene 46 14.845 46 12.962
n-propylbenzene 47 15.966 47 14.124
1,3,5-trimethylbenzene 48 16.565 48 14.711
decane 49 17.166 45 12.369
1,2,4-trimethylbenzene 50 17.586 49 15.904
butylbenzene 51 19.739 51 17.732
tetradecane 52 29.806 52 24.950
1. tert-butyl nitrite
2. iso-butyl nitrite
3. sec-butyl nitrite
4. n-butyl nitrite
5. iso-amyl nitrite
5
4
3
2
1
min. 2 6 4
1
5
4
3
2
30m, 0.53mm ID, 3.0µm Rtx®-BAC1 (cat.# 18001)
30m, 0.53mm ID, 2.0µm Rtx®-BAC2 (cat.# 18000)
8 10
1.0mL headspace sample
Oven temp.: 40°C (hold 5 min.) to 240°C @ 5°C/min.
Inj. & det. temp.: 240°C
Carrier gas: He
Linear velocity: 65cm/sec.
1. tert-butyl alcohol
2. iso-butyl alcohol
3. sec-butyl alcohol
4. n-butyl alcohol
5. iso-amyl alcohol
min. 2 6 4 8 10
5
4
3
2
1
5
4
3
2
1
Figure 3 Screen for and confirm alkyl nitrites and metabolites on
Rtx®-BAC1 and Rtx®-BAC2 columns.
30m, 0.53mm ID, 3.0µm Rtx®-BAC1 (cat.# 18001) and
30m, 0.53mm ID, 2.0µm Rtx®-BAC2 (cat.# 18000).
1.0mL headspace sample
Oven temp.: 40°C (hold 5 min.) to 240°C @ 5°C/min.
Inj. & det. temp.: 240°C
Carrier gas: He
Linear velocity: 65cm/sec.