Rtx015-16-628

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GC COLUMNS | GENERAL-PURPOSE COLUMNS
Rt®-2560
Rt®-2560 Column (fused silica)
(highly polar phase; biscyanopropyl polysiloxane—not bonded)
• Application-specific column for cis/trans FAMEs.
• Stable to 250 °C.
Because the Rt®-2560 stationary phase is not bonded, it should not be solvent rinsed.
similar phases
HP-88, CP-Sil 88, SPB-2560
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2324
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26 27 28
FAMEs (NLEA Mix) on Rt®-2560
GC_FF00651
Column Rt®-2560, 100 m, 0.25 mm
ID, 0.20 µm (cat.# 13199)
Sample NLEA FAME mix
(cat.# 35078)
Diluent: Methylene chloride
Conc.: 30 mg/mL total FAMEs
Injection
Inj. Vol.: 1 µL split (split ratio 100:1)
Liner: Splitless (4 mm ID)
(cat.# 20814)
Inj. Temp.: 225 °C
Oven
Oven Temp.: 100 °C (hold 4 min) to
240 °C at 3 °C/min (hold
10 min)
Carrier Gas H2 , constant flow
Flow Rate: 1.2 mL/min
Detector FID@ 250 °C
Peaks
1. C4:0 methyl butyrate
2. C6:0 methyl hexanoate
3. C8:0 methyl octanoate
4. C10:0 methyl decanoate
5. C11:0 methyl undecanoate
6. C12:0 methyl laurate
7. C13:0 methyl tridecanoate
8. C14:0 methyl myristate
9. C14:1 methyl myristoleate (cis-9)
10. C15:0 methyl pentadecanoate
11. C16:0 methyl palmitate
12. C16:1 methyl palmitoleate (cis-9)
13. C17:0 methyl heptadecanoate
14. C18:0 methyl stearate
15. C18:1 methyl elaidate ( trans-9)
16. C18:1 methyl oleate (cis-9)
17. C18:2 methyl linoelaidate ( trans-9,12)
18. C18:2 methyl linoleate (cis-9,12)
19. C20:0 methyl arachidate
20. C20:1 methyl eicosenoate ( cis-11)
21. C18:3 methyl linolenate (cis-9,12,15)
22. C22:0 methyl behenate
23. C22:1 methyl erucate (cis-13)
24. C23:0 methyl tricosanoate
25. C24:0 methyl lignocerate
26. C20:5 methyl eicosapentaenoate
( cis-5,8,11,14,17)
27. C24:1 methyl nervonate (cis-15)
28. C22:6 methyl docosahexaenoate
( cis-4,7,10,13,16,19)
An Rt®-2560 column provides the
resolution of cis & trans FAMEs
needed for AOAC Method 996.06.
100-Meter
ID df temp. limits cat.# price
0.25 mm 0.20 µm 20 to 250 °C 13199