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GC COLUMNS |
APPLICATION-SPECIFIC COLUMNS
Clinical, Forensic, & Toxicology
Rtx®-BAC Plus 1 Columns
(fused silica)
Rtx®-BAC Plus 1/Rtx®-BAC Plus 2 Columns
• Optimized column selectivities guarantee resolution of ethanol, internal standards,
and frequently encountered interferences.
• Robust and reproducible column chemistry ensures longer column lifetime and
consistent results.
• Stable to 260 °C.
These application-specific columns for blood alcohol analysis baseline separate all
critical compounds—including ethanol, methanol, acetone,
tert
-butanol, acetalde-
hyde, isopropanol, and
n
-propanol—in less than 2 minutes. Every Rtx®-BAC Plus 1
and Rtx®-BAC Plus 2 column is qualified with a test mix containing these important
BAC target compounds to ensure reproducibility.
These columns baseline separate all blood alcohol compounds in blood, breath, or
urine, in less than 2 minutes, under isothermal conditions. Isothermal analysis in-
creases productivity by eliminating the need for oven cycling. Confirmation is easily
achieved with this tandem set because there are two elution order changes between
the columns.
Rtx®-BAC Plus 2 Columns
(fused silica)
Blood Alcohol Analysis
Blood Alcohol Analysis on Rtx®-BAC Plus 1 and Rtx®-BAC Plus 2
Time (min)
0.5
0.75
1
1.25
1.5
1.75
2
2.25
2.5
2.75
1
2
3
4
5
6
7
Blood Alcohol Analysis on Rtx®-BAC Plus 1
GC_CF1153
Column
Rtx®-BAC Plus 1, 30 m, 0.32 mm ID, 1.8 µm (cat.# 18004)
Sample
BAC resolution control standard n-P (cat.# 36010)
BAC resolution control standard t-B (cat.# 36011)
Diluent:
Water
Conc.:
50 µL of each standard were diluted in 900 µL water in a
20 mL headspace vial.
Injection
Headspace-loop split (split ratio 50:1)
Liner:
1 mm ID straight inlet liner (cat.# 20972)
Headspace-Loop
Inj. Port Temp.:
200 °C
Instrument:
Tekmar HT3
Inj. Time:
3 min
Transfer Line
Temp.:
125 °C
Valve Oven Temp.: 125 °C
Standby flow
rate:
50 mL/min
Sample Temp.:
60 °C
Sample Equil.
Time:
5 min
Vial Pressure:
30 psi
Pressurize Time: 1 min
Loop Pressure:
20 psi
Loop Fill Time:
1 min
Oven
Oven Temp.:
40 °C (hold 3 min)
Carrier Gas
He, constant flow
Linear Velocity:
80 cm/sec @ 40 °C
Detector
FID@ 240 °C
Make-up Gas
Flow Rate:
30 mL/min
Make-up Gas
Type:
N
2
Instrument
Agilent/HP6890 GC
Notes
Headspace concentrator courtesy of Teledyne Tekmar,
Mason, OH.
Peaks
t
R
(min)
Conc.
(µg/mL)
1. Methanol
0.912
100
2. Acetaldehyde
0.967
100
3. Ethanol
1.158
100
4. Isopropanol
1.420
100
5. Acetone
1.548
100
6.
tert
-Butanol
1.688
50
7. 1-Propanol (
n
-Propanol)
1.969
50
Time (min)
0.5
0.75
1
1.25
1.5
1.75
2
2.25
2.5
2.75
1
2
3
4
5
6
7
Blood Alcohol Analysis on Rtx®-BAC Plus 2
GC_CF1154
Peaks
t
R
(min)
Conc.
(µg/mL)
1. Acetaldehyde
0.809
100
2. Methanol
0.861
100
3. Ethanol
1.034
100
4. Acetone
1.119
100
5. Isopropanol
1.181
100
6.
tert
-Butanol
1.304
50
7. 1-Propanol (
n
-Propanol)
1.658
50
free literature
Rtx®-BAC Plus 1 and
Rtx®-BAC Plus 2 Columns
Advanced Technology for Fast,
Reliable Measurement
of Alcohol in Blood
Download your
free copy from
lit. cat.#
CFBR1538-UNV
www.restek.com30-Meter
ID df
temp. limits
cat.#
price
0.32 mm 1.80 µm -20 to 240/260 °C 18004
0.53 mm 3.00 µm -20 to 240/260 °C 18005
30-Meter
ID df
temp. limits
cat.#
price
0.32 mm 0.6 µm -20 to 240/260 °C 18006
0.53 mm 1.0 µm -20 to 240/260 °C 18007
similar phases
DB-ALC1, ZB-BAC1
DB-ALC2, ZB-BAC2