30
www.restek.comOver 10,000 Injections of Lipidomics on Rxi®-5ms
Rxi®-5ms Columns
(fused silica)
(low-polarity phase; Crossbond® diphenyl dimethyl polysiloxane)
• General-purpose columns for semivolatiles, phenols, amines, residual solvents,
drugs of abuse, pesticides, PCB congeners (e.g., Aroclor mixes), solvent impurities.
• Most inert column on the market.
• Tested and guaranteed for ultra-low bleed; improved signal-to-noise ratio for better
sensitivity and mass spectral integrity.
• Temperature range: -60 °C to 330/350 °C.
• Equivalent to USP G27 and G36 phases.
Rxi®-5ms Structure
CH
3
CH
3
Si
95%
O
Si
5%
O
Similar to: (5%-phenyl)-methylpolysiloxane
free literature
Rxi® Columns
Lower Detection Limits with Ground-
Breaking Column Technology
Download your .
free copy from
lit. cat.# .
GNFL1173A
www.restek.comGC_PH01053
Column
Rxi®-5ms, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13423)
Sample
Lipid plasma extract as trimethylsilyl derivatives
Injection
Inj. Vol.:
1 µL split (split ratio 10:1)
Liner:
4 mm split liner gooseneck with wool
Inj. Temp.:
310 °C
Oven
Oven Temp.:
250 °C (hold 1 min) to 320 °C at 30 °C/min (hold 1.6 min)
Carrier Gas
He, constant flow
Flow Rate:
1.0 mL/min
Detector
MS
Mode:
SIM
Transfer Line Temp.: 330 °C
Ionization Mode:
EI
Peaks
m/z
1. Coprostanol
370.40
2. d5-cholestanol (IS)
220.20
3. 7
α
-hydroxycholesterol 456.40
4. Cholestanol
306.30
5. d5-epicholestanol (IS) 220.20
6. 7-Dehydrocholesterol
325.25
7. Desmosterol
343.25
8. Lathosterol
458.35
9. d4-lathosterol (IS)
462.40
10. Campesterol
382.35
11. 4-Cholestenone
382.30
12. Stigmasterol
394.15
13. Lanosterol
393.35
14.
β
-Sitosterol
396.35
15. d7-
β
-sitosterol (IS)
403.45
GC COLUMNS |
HIGH-PERFORMANCE Rxi® COLUMNS
Rxi®-5ms
15-Meter
30-Meter
60-Meter
ID df
temp. limits
cat.#
price
cat.#
price
cat.#
price
0.25 mm 0.25 µm -60 to 330/350 °C
13420
13423
13426
0.40 µm -60 to 330/350 °C
13481
0.50 µm -60 to 330/350 °C
13435
13438
13441
1.00 µm -60 to 330/350 °C
13450
13453
13456
0.32 mm 0.25 µm -60 to 330/350 °C
13421
13424
13427
0.50 µm -60 to 330/350 °C
13436
13439
13442
1.00 µm -60 to 330/350 °C
13451
13454
13457
0.53 mm 0.25 µm -60 to 330/350 °C
13422
13425
0.50 µm -60 to 330/350 °C
13437
13440
1.00 µm -60 to 330/350 °C
13452
13455
1.50 µm -60 to 330/350 °C
13467
13470
12-Meter
20-Meter
25-Meter
50-Meter
ID df
temp. limits
cat.#
price
cat.#
price
cat.#
price
cat.#
price
0.18 mm 0.18 µm -60 to 330/350 °C
13402
0.30 µm -60 to 330/350 °C
13409
0.36 µm -60 to 330/350 °C
13411
0.20 mm 0.33 µm -60 to 330/350 °C
13497
13498
13499
similar
phases
HP-5ms SemiVolatiles, HP-5ms, HP-5msUI,
DB-5, Ultra-2, CP-Sil 8 CB, ZB-5, ZB-5msi
Stringent quality testing ensures consistent
performance, column to column and .
injection to injection.