175
LC COLUMNS
Columns for Bioseparations
Viva HPLC Columns: 3 or 5 µm particles; 300 Å pore size
• Excellent for separating peptides or proteins.
• Rugged, spherical particles with 300 Å pore size.
• High proportion of pore/surface area available to large molecules.
Viva columns are based on a wide pore material we designed for optimal large-molecule separations. In developing Viva silica, we
found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distribu-
tions about this mean, with a significant portion of their pore volume falling below 150 Å. This means a large portion of the surface
area is unavailable to larger molecules. Viva columns have a narrow distribution around the mean pore size, permitting a larger
portion of the silica surface to play a role in the separation.
Restek-
manufactured
silica
Restek-
manufactured
silica
Column Characteristics:
particle size:
3 µm or 5 µm,
spherical
pore size:
300 Å
carbon load:
9%
end-cap:
yes
pH range:
2.5 to 8
temperature limit: 80 °C
USP phase code:
L1
phase category:
C18,
octadecylsilane
ligand type:
monomeric C18
Si
CH
3
CH
3
O
C18
Column Characteristics:
particle size:
5 µm, spherical
pore size:
300 Å
carbon load:
5%
end-cap:
yes
pH range:
2.5 to 8
temperature limit: 80 °C
USP phase code:
L7
phase category:
C8, octylsilane
ligand type:
monomeric C8
Si
CH
3
CH
3
O
C8
Restek-
manufactured
silica
Chromatographic Properties
The general-purpose Restek® C18 is a conventional monomeric octadecylsilane column suitable
for analyses of a wide range of compounds from acidic through slightly basic.
Viva C18 Columns
(USP L1)
Chromatographic Properties
Our C8 is a conventional monomeric octylsilane column offering a shorter alkyl chain to pro-
vide less hydrophobic retention and improved basic peak shape over a traditional C18 phase.
Like our C18, this general-purpose Restek® C8 is suitable for a wide range of compounds from
acidic through slightly basic.
Viva C8 Columns
(USP L7)
1.0 mm ID
2.1 mm ID
3.0 mm ID
4.6 mm ID
Length
cat.#
price
cat.#
price
cat.#
price
cat.#
price
3 µm Columns
30 mm
9514331
9514332
951433E
9514335
50 mm
9514351
9514352
951435E
9514355
100 mm
9514311
9514312
951431E
9514315
150 mm
9514361
9514362
951436E
9514365
5 µm Columns
30 mm
9514531
9514532
951453E
9514535
50 mm
9514551
9514552
951455E
9514555
100 mm
9514511
9514512
951451E
9514515
150 mm
9514561
9514562
951456E
9514565
200 mm
9514521
9514522
951452E
9514525
250 mm
9514571
9514572
951457E
9514575
1.0 mm ID
2.1 mm ID
3.0 mm ID
4.6 mm ID
Length
cat.#
price
cat.#
price
cat.#
price
cat.#
price
5 µm Columns
30 mm
9513531
9513532
951353E
9513535
50 mm
9513551
9513552
951355E
9513555
100 mm
9513511
9513512
951351E
9513515
150 mm
9513561
9513562
951356E
9513565
200 mm
9513521
9513522
951352E
9513525
250 mm
9513571
9513572
951357E
9513575