133
GC COLUMNS |
PACKED/MICROPACKED COLUMNS
Bonded Stationary Phases
Restek’s packed columns deliver the
1
-
2
-
3
PUNCH!
1. Bonded stationary phases mean short .
conditioning times, low bleed, and .
unsurpassed column lifetimes.
2. SilcoSmooth® tubing provides the inertness
of glass and the durability of stainless steel.
3. Excellent retention time reproducibility for
reliable, consistent results!
Bonded Packed Column Stationary Phases
• Short conditioning times.
• Reproducible bonded phase selectivity.
• Low bleed levels.
• Longer column lifetimes.
• Unsurpassed inertness for active compounds.
Bonded phases are used in capillary columns because they provide a dramatic increase
in column quality. To truly bridge the gap between traditional packed columns and
capillary columns, it was necessary to develop bonded liquid phases for packed col-
umns. Packed column chromatographers can expect shorter conditioning times, lower
bleed, and longer column lifetimes by using Restek bonded phase packed columns.
Bonded phases also last much longer than nonbonded phases. Bonded phases are more
resistant to oxidation than nonbonded phases because of the stronger intermolecular
forces produced by cross-linking. Because the material is thoroughly cross-linked, the
phase will not migrate or puddle, as often happens with nonbonded phases. Figure 1
shows a comparison of a bonded and a nonbonded methyl silicone column after 170
temperature cycles. The results show the impressive durability of bonded phases.
Figure 1: Bonded packed columns exhibit longer lifetime than nonbonded packed columns.
GC_PC00369
Column
Rtx®-1 Sim Dist 2887, SilcoSmooth® Tubing, 25 inches,
1
/
8
in. OD, 2 mm ID (cat.# 80000-800)
Sample
1-12% (w/w) each component
ASTM D2887-01 calibration mix (1% each listed analyte in CS
2
) (cat.# 31674)
ASTM D2887-01 calibration mix (5% each, neat) (cat.# 31675)
Injection
Inj. Vol.:
1.0 µL packed not on-column
Inj. Temp.:
350 °C
Oven
Oven Temp.:
35 °C to 350 °C at 10 °C/min (hold 5 min)
Carrier Gas
He, constant flow
Flow Rate:
25 mL/min
Detector
FID@ 350 °C
Notes
FID sensitivity: 256 x 10
-11
AFS
Peaks
1. Pentane
2. Hexane
3. Heptane
4. Octane
5. Nonane
6. Decane
7. Undecane
8. Dodecane
9. Tetradecane
10. Hexadecane
11. Octadecane
12. Eicosane
13. Tetracosane
14. Octacosane
15. Dotricontane
16. Hexatricontane
17. Tetracontane
18. Tetratetracontane
Analytes cannot
be quantified
Low bleed after 170
cycles to 350 °C!
Nonbonded Methyl Silicone
Bonded Rtx®-1
Equivalent Liquid Phases
BP-1, CC-1, CP-Sil 5CB, DB-1, DC-200, GE-SF-96, HP-1, HP-101, OV-1, OV-101,
Rtx-1
RSK-150, RH-1, SE-30, SP-2100, SPB-1, UCC W-98, G2, G1
Rtx-5
BP-5, CB-5, CC-5, CP-Sil 8CB, DB-5, HP-5, OV-73, SE-52, SE-54, SPB-5, Ultra-5, G27, G36
Stabilwax
BP-20, CP-Wax, CW-20, DB-Wax, HP-Innowax, PE-Wax, Supelcowax-10, G16