Basic HTML Version
5
Revision 2.0
1/1/02
3.1 The end of the tubing is vertically cut with a knife or a tubing cutting device.
CAUTION! - Do not cut with scissors to prevent squeezing. It is essential to cut the tubing
vertically, as the cut edge is part of the sealing area!
3.2 The fixing screw is drawn over the tubing.
3.3 .3 The locking rings is drawn over the tubing with the conical side against the banjo
bolt. If it is not possible to mount the locking ring, the conical end is widened
carefully with a suitable arbor (e.g. a scribing iron).
3.4 Now the fitting can be inserted in the wanted position. The seal is pressed onwards,
while the screws are fastened until pressure is felt; then they are fastened for another
½ turn.
CAUTION!: - When the fittings are screwed into the teflon® thread it should be taken
care that the fittings are screwed down straightly and are fastened carefully to prevent damage
to the teflon® thread.
4.0 ELIMINATION OF DEAD VOLUME:
A dead volume which might occur at the column inlet can be remedied simply without the
need to open the column:
4.1 Turn off pump.
4.2Turn the divisible column lock counterclockwise.
CAUTION: - The piston should touch only the surface of the stationary phase. If it is
pressed into the stationary phase, the packing may be destroyed.
5.0 OPERATION OF THE COLUMN:
Putting into operation
Pistons (3 and 7), frits and column body (5) must be cleaned carefully before first use and before
each new packing. In some cases it might be useful to dismount the column and wash these parts
in a sonic bath for several minutes. After cleaning all parts must be rinsed with bio-distillated
water and mounted as described under chapter 1, part 2. All parts must be free of dust and par-
ticles!
Be sure that the pistons (3 and 7) are inserted carefully into the column body (5): If not intro-
duced absolutely axial, the piston seals might be damaged.
For operating the column it is connected to an appropriate chromatography system or pump.
Chose tubing and tubing diameter according to the solvents and flow rates to be applied to the
column. The preferred flow direction of the column should be from variable piston to fixed piston.