Page 38 - Ultra II HPLC ColumnPresentation - Restek

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0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
min
0
10
20
30
40
50
60
mAU
254nm,4nm (1.00)
1. Ketoprofen
2. Piroxicam
3. Sulindac
4. Diclofenac
Ultra II Biphenyl 2.2um, 50 x 3.0mm
Mobile Phase: 30:70 A:B
A: 20mM K
x
(PO
4
)
y
, pH 2.5
B: Methanol
Flow: 1.2 mL/min
Temp.: 40 °C
Det.: UV @ 254 nm
Shimadzu Prominence UFLC
XR
Injection: 5 µL, 15 µg/mL each
Sample Diluent: Methanol
NSAIDs on Ultra II
®
Biphenyl
a
®
1
2
3
4
38
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