Page 21 - Ultra II HPLC ColumnPresentation - Restek

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estriol
estradiol
testosterone
estrone
ethynyl
estradiol
norethindrone
Preliminary Separation
3µm, 50mm x 4.6mm Column
Mobile Phase – 50:50
Water:Acetonitrile
Flow rate: 1.5 mL/min, isocratic
UV detection @ 254nm
Temperature: Ambient
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Example: Steroid Hormones
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