✻
C18 with both
C18 with both
silanol
silanol
activity control and full end
activity control and full end
-capping is effective
capping is effective
separation of polar compounds.
separation of polar compounds.
■
Separation of organic acid
Separation of organic acid
(
Sunrise C18
Sunrise C18
)
■
Separation of nucleic acid bases
Separation of nucleic acid bases
(
Sunrise C18
Sunrise C18
)
0 1 2 3 4 5 6 7 8 9 10 11 12
0 1 2 3 4 5 6 7 8 9
Column size: 4.6x150 mm
Mobile phase: 20mM PBS (pH4.
Flow rate: 1.0 mL/min
Temperature: 40
°
C
Sample:
1 = cytosine
2 = uracil
3 = thymidine
4 = thymine
Column size: 4.6x150 mm
Mobile phase:2/98=CH
3
CN
/0.1% phosphoric acid
Flow rate: 1.0 mL/min
Temperature: 40
°
C
Sample:
1 = formic acid
2 = acetic acid
3 = propionic acid
retention time / min
3
1 2
3
1
2
retention time / min
4
Sunrise C18 and C18
Sunrise C18 and C18
-SAC
SAC
Silanol Activity Controlled C18 HPLC Column
Silanol Activity Controlled C18 HPLC Column
◆
Sunrise series
Sunrise series
create an unique separation
create an unique separation
■
comparison of selectivity for basic compounds
comparison of selectivity for basic compounds
Column size: 4.6x150 mm
Mobile phase:
50/50=CH
3
CN/20 mM PBS pH4.5
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = uracil
2 = propranolol
3 = nortriptyline
4 = amitriptyline
5 = toluene
0
5
10
15
20
retention time / min
B) Sunrise C18
B) Sunrise C18
-SAC
SAC
2
1
5
3
4
2
1
5
3
4
A) Sunrise C18
A) Sunrise C18
■
comparison of peak shape and retention
comparison of peak shape and retention
Column size: 4.6x150 mm
Mobile phase:
10/90=CH
3
CN/20mM H
3
PO
4
Flow rate: 1.0 mL/min
Sample: 1 = 8-quinolinol (oxine)
2 = caffeine
1
1
0 2 4 6 8 10 12 14 16 18 20 22
retention time / min
2
2
B) Sunrise C18
B) Sunrise C18
-SAC
SAC
A) Sunrise C18
A) Sunrise C18
✻
Effectiveness of
Effectiveness of
silanol
silanol
activity control: Comparison between
activity control: Comparison between
Sunrise
Sunrise
C18
C18
and
and
C18
C18
-SAC
SAC
Sunrise C18 is the so-called fully end-capped C18
column. It shows the same separation behavior as a
conventional C18 column.
On the other hand, Sunrise C18-SAC shows
hydrogen bond and ion-exchange interactions
based on a residual silanol on the silica support in
addition to reversed-phase separation. For example
Sunrise C18 column separates a basic
compound
similarly as a conventional C18, while Sunrise C18-
SAC makes retention of a basic compound be
large because an ion-exchange interaction works
although a non-ionic compound shows the almost
same retention on both Sunrise C18 and C18-SAC.
Furthermore, Sunrise C18-SAC shows large retention
for a polar compound such as caffeine.
Sunrise C18 is bonded with octadecylsilane on pure
silica gel (average pore size: 12nm, specific surface
area: 340m
2
/g), and end-capped after silanol
activity control. Final carbon content of Sunrise C18
is 15%.
Ligand density of Sunrise C18 is intentionally rather
low and uniformity of ligands is high, so that it shows
enough retention, even if a mobile phase with a
low organic solvent content is used, and good
peak shape for a polar compound.