Sunrise C18 and C18
Sunrise C18 and C18
-SAC
SAC
Silanol
Silanol
Activity Controlled C18 HPLC Column
Activity Controlled C18 HPLC Column
Sunrise C18
Sunrise C18
Sunrise C18
Sunrise C18
-SAC
SAC
•The
The
“1st Choice
1st Choice
” column
column
as a fully end
as a fully end
-capped C18 column
capped C18 column
•Full end
Full end
-capping
capping
after
after
silanol
silanol
activity control
activity control
•Reducing
ducing
adsorption of
adsorption of
a basic compound
a basic compound
extrem
extrem
ely
ely
•A
A
good peak shape for a
good peak shape for a
metal
metal
cheleting
cheleting
comp
comp
ound
ound
•Widely available for general reverse
Widely available for general reverse
d-phase
phase
separation
separation
•The "2nd Choice" column which
The "2nd Choice" column which
take
take
s advantage of
advantage of
effective
effective
silanol
silanol
groups
groups
interaction
interaction
•Reducing
Reducing
silanol
silanol
groups with high
groups with high
adsorption activ
adsorption activ
ity
ity
•The new
The new
separation
separation
mechanism
mechanism
including hydrogen bond
including hydrogen bond
and ion
and ion
-exchange interaction
exchange interaction
•Effective for
ffective for
separation of
separation of
a basic compound
a basic compound
and
and
a polar
a polar
compound
compound
•Different selectivity and
Different selectivity and
improvement
improvement
of separation
of separation
without
without
changing a mo
changing a mo
bile phase
bile phase
■
The elution order of pyridine
The elution order of pyridine
It is generally said that residual silanol groups on
a stationary phase such as C18 (ODS) causes
absorption or peak tailing for a sample.
Especially silanol groups near a hydrophobic
site don
’
t solvate with water completely, so that
they show high absorption for basic compounds.
Its peak shows terribly tailing. Several end-
capping techniques have been developed to
solve these problems for many years.
◆
New generation reversed
New generation reversed
-phase utilized silanol groups
phase utilized silanol groups
◆
Feature
Feature
of Sunrise series
of Sunrise series
1st generation
1st generation
4th generation
4th generation
3rd generation
3rd generation
2nd generation
2nd generation
Pellicular type
Bonded type
High carbon and
end-capped type
Hybrid type
(Post-X
Sunris
C18-SA
Highly end-
capped type
5th generation
5th generation
Silanol Activity
Control
Technology
Sunrise
ChromaNik developed the technique that
decreased only silanol groups with high
absorption activity to a basic compound and
remained effective sailnol groups on the
stationary phase. Silanol activity control and no
end-capping led the existence of silanol groups
with high hydration which created a new and
unique reversed-phase separation mode
including hydrogen bond and ion-exchange
interaction. Furthermore, silanol activity
controlling, then end-capping technique
improved a peak shape of a basic compound
exceedingly.
■
Silanol group and peak tailing
■
Silanol activity control technology
Silanol
Silanol
activity control
ctivity control
O
Si
H
O
Si
H
O
Si
O
O
Si Si
H
O
Si
H
O
Si
O
O
Si Si
Si
Silanol groups
with high
absorption activity
Changeover to
siloxane bond
Silanol Activity
Control
Technology
C) Sunrise C18
C) Sunrise C18
-SAC
SAC
(non
(non
end
end
-capped)
capped)
1
1
3
3
0
5
10
15
20
retention time / min
(2)
(2)
(2)
(2)
A) Sunrise C18
A) Sunrise C18
(end
(end
-capped)
capped)
Column size: 4.6 X 150mm
Mobile phase:
30/70= CH3OH/H2O
Sample: 1 = uracil
(2) = pyridine
3 = phenol
B) Conventional no
B) Conventional no
end
end
-capped
capped
C18
C18
1
(2)
(2)
3