8
Sample Prep: HMFStandard byHPLC
HMF
Column: UltraC18 (RestekCorp.), 250mm x 4.6mm, 5µm
MobilePhaseA: 90:10water:methanol, 10mMammonium formate
MobilePhaseB: 10:90water:methanol, 10mMammonium formate
Gradient: 0-5minat 100%A, to100%Bat 10min, 10min. hold
Flow:
0.5mL/min.
Temperature:
ambient
Detector:
UV@280nm
InjectionVolume: 10µL
