OptimizingMobilePhases forAPI-MS
• Ion-Pair Agent Alternatives
¾
Usehighly bases-deactivated silica columns
¾
Use low pH (3-4) to reduce tailing
¾
Use columns that retain basedon polar
interactions (e.g. CN, IBD)
• Using Ion-Pair Reagents
¾
Use lowamounts (<0.02%)
¾
Use post-column addition to negate theeffect
of the ion-pair agent
