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& a HI-TECH “Resource” > 4 U !
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but see “Scams” > New”Phishes”
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CHROMTECH : Specialists in Chromatography Chromatography Fluid Transfer Products : & FOR ! All Your Laboratory Needs ! - Importers & Distributors in AUSTRALIA
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![](./a_ArrowLine_1.gif) |
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available . . . in Syringe Filters
![SyringeFilters SyringeFilters](./htm-button_53.jpg) 0.45 and 0.22um 13, 25, 33mm Diameter CA, PES, Nylon, PP, PVDF, PTFE Non-sterile STERILE (? ASK ! ) Normal single filter GlassFibre
![SyringeFilter GassFibre SyringeFilter GassFibre](./htm-button_53.jpg) preFilter 1um / Main multiLayer 10,1.0,0.7um / Main
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Whatman GD/X PVDF
![Whatman GD/X Whatman GD/X](./htm-button_53.jpg) “accepted state of Art” obsolete w/PES (New)
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Membrane Disc Filters
![Membrane FilterDisc Membrane FilterDisc](./htm-button_6.jpg) BottleFilters and Glass Funnels 0.45um Nylon, PVDF, Glass Fibre 1um 47mm Diam
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Filtration Speed ? - micron size of filter media 0.45um > 0.22um >>0.1um - surface area of media 33 > 25 > 13mm Syringe Filter 90mm VacuCap > 70mm > 50mm BottleTop Filter - source of pressure Positive pressure OR Vacuum
- VISCOSITY
Increase Temperature > 20C+ approx x 2 as Fast but depends on Max media Temp limit
- Use a Courser
pre-Filter to trap large particles ?) >protects the main membrane
- FILTER MEDIA Type
try match the polarity with that of the matrix fluid !
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how long is a piece of string” . . . it All depends ! . it IS VERY sample dependant !. . . ALL Trial and Error ! required - particulate matter; how dirty and NOT just visible dirt but from product manufacture re crude paper filter methods - “invisible fibres” etc Factors : Viscosity . . . temperature dependant / dilution if possible oils/solvents warm fluid . . . in extreme cases use Syringe Pump and heated Syringe Jacket Chemical Compatability / wettability sample fluid & filtration media and equipment Specifics : Teflon and PVDF may need to be prewetted - isopropanol Structure of different OEM filter media for same nominal pore size may be different random fibres vs mesh type : pore size is a average pore size measurement and porosity depends on dispersion of pores about a mean size
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some Equipment Limitations BottleTop Filter
preferred for aqueous media and medium Vac < 250mm Hg - higher vacuum can collapse plastic vacuum bottles - plastic bottles can be softened with “oils” particularly if media has to be warmed - aggressive solvents can soften filter housing material (PP/syringe filters ) - filter fibres can swell ( close off pores ) if filter media and fludis are ”less” compatible - match hydrophyllic with hydrophyllic and hydrophobic with hydrophobic - non polar oils use PP, Teflon PVDF - essential oils/ fatty acids ester oil PVDF, Nylon perhaps - aqueous solution, high speed PES (polyetherSulfone), Nylon Glass Filtration Equipment
- requires Heavy Duty Glass receiver > But Take CARE ! - more expensive . . . initial cost - very robust ( but NOT unbreakable ) - safer for higher vacuum if required ( use safety shield as glass can implode if scratched or cracked ) - can be easily cleaned . . . sterilised if needed - will handle most aggressive fluids and solvents Vacu-Cap
- for large batches ( 100ml to 250ml )of fast fluids, generally aqueous and often HandVacuum pump adequate and controllable / managable. tedious > motorised VacPump ( for larger OR slow /viscous mixes 0.1-0.2um SFs ! )
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Vacuum System Hand Vacuum Pump : can develop higher vacuum at slow filtration speeds and collapse plastic filtration vacuum bottles - use small filter flasks less pumping required - can be tedious/tiresome for large batches and fast flow fluids Motorised Vacuum Pump
: for large batches, vacuum can be regulated and filtration pumps 250mm HG is adequate for most fluids - takes tedium out of slow filtration fluids ie set and “forget”
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Syringe Filter
![SyringeFilter SyringeFilter](./htm-button_22.jpg) - pressurised by thumb pressure ( syringe dispenser ) 33mm for 50 to 200ml, 25mm 25 to 100ml, 13ml 5 to 25ml - depending for large through puts - tedious and thumb soreness are issues - slow fluids use a Syringe Pump
- for viscous fluids either dilute with a solvent or use a Syringe Heater jacket ( can easily develop 100psi ) Syringe Filters are rated only to 50-75psi max Use Heavy Duty Glass Syringe or SS syringe if necessary - come in a variety of filter materials; CA, Nylon, PES, PP, PVDF, Teflon
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Prefilters
- are used to speed up flow and SF capacity coarser layer traps large particles and helps protect the main filter material - Glass Fibre preFilter usually 1umlayer built-in ( Membrane Solutions ) - multiLayer preFilter : 10um, 1um , 0.7um layers then main filter 0.45um or 0.22um - Glass Fibre preFilter can also be used with Filter Funnel systems - either stand-alone GF Filter or separate membrane filter added on top of main filter / combined Whatman GD/X PVDF Syringe Filters . . . are AGAIN now available from Chromalytic Tech > obsolete(?) with New PES ( at least for mid-polarity Oils” ! )
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re “Sterility” NOTE : TGA IS Pedantic !
This is not to be regarded as advice re TGA implications - see Chromalytic DISCLAIMER Sterile equipment; filters etc WILL NOT necessarily sterilise unsterile fluids Where originally STERILE Fluids need to be filtered AND Sterility needs to be maintained then ALL filtration equipment including Filter Media must be purchased sterile or suitably sterilised in-situ and recommended handling proceedures / asceptic conditions a bit of “common sense” is ALSO required !“gamma” sterilisation may tend to disintegrate some “rubber” Stoppers’ > but CHECK First !.
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some methods ! disinfectants, boiling water, dry heat 300deg C ( borosilicate glass ) (steam) autoclaving ( 130degC ) industrial , ethylene oxide gas; gamma irradiation . . . with clean room handling/packaging to strict TGA requirements . . . for pharmaceuticals and medical use MicroFiltration : 0.45um sterile filters - only part successful ( arbitrary 60% ) 0.22um sterile filters much more effective ( 90% ) 0.1um sterile filters - > 98% - but NO GUARANTEES !
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Application development testing or small amounts of fluid per batch ( 200ml max )
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Syringe Filter MS
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1 with Luer Lok Syringe 13,25,33mm Diameter 2 Reusable Cartridge Filter Holder - disposable 25mm Disc Filters
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Step 1 consider material compatability - filtration equipment and fluid Expect variations in Fluid quality, contamination - use pure fluids QA/QC issues Step 2 test/evaluate Syringe Filters hydrophyllic vs hydrophobic water solutions : Nylon/PES to oils PP/PVDF/Teflon (see Filtration Media Hints) microfiltration small qtys 13mm, then 25mm or 33mm ( more expensive )
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if slow
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due to large particulates
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Consider preFilter (1) 1um Glass Fibre (2) multi-Layer
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viscous solutions
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- try warming or if possible dilution with solvent subsequent evaporation or sparging of solvent Oils - NO open flame heating - use a hot plate
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too high a pressure on Your thumb
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- consider use of a Syringe Pump / with heater option low pressure - Plastic NormJect Syringes higher pressure/heated - se heavy duty glass syringe or SS syringe - SF max is 75psi
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1 Initially use 0.45um as the main filter 2 Use the finer 0.22um (0.1um) in a subsequent / separate filtration step
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Larger Quantities
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Aqueous / sterile ?
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Hand VacPUMP and Bottletop Filter MS and Nalgene VacuCap for mobile fluid fast throughput / with Hand VacuumPUMP
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Oils / aqueous
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Glass Filtration Equipment with HandVacPUMP - motorised VacPUMP - easier over prolonged filtration periods - multi-manifold /funnel systems are available
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Chromatography Filtration see Sample Filtration products
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to protect instruments ! eg HPLC Pumps / GC Injection ports
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dirty samples
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1 consider 13mm SFs - check for sample loss QA/QC 2 Single Step Filter Vials PDF
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FILTRATION Products : PIC Gallery> GOOGLE SEARCH PIcs > PDF LINKs
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> a TECH Resource for Analytical Chemists ! CT Chromatogrphy > PIC Gallery > via the “courtesy” of ”Our” GOOGLE SEARCH PICs >PDF LINKs > Good IDEAS : Gadjets
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We’d Welcome YOUR e-Mail /chat Response > but YOUR “Hi-TECH“ “Feedback” is also most Welcome !
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Corrections ! > ALL ( > MANY ) “HTM” links with “ “ or a “%20” ( space ) in the URL now are “auto-fixed” on-line by NOF15 NOT with “%20” but with “ -” ( hypen ) instead & are NOW W3C / HTML5 OK ! “ _ “ ( underscore ) are also accepted ! with NO dreaded “404” errors !* FlipHTML5 & PDFs ar NOT effected by this “farcical”>”anomaly” !
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NOW ? > (“simlpy” ] use TAWK
from ~31Mar24+ but still ? > & evolving ! albeit TAWK “HELP”itself Is Still a bit “farcical” ! Re AI > garbage-IN > OUT ! . . . but still 2-5year away to minimise obvious “scams > “phishing” etc? Until ALL such OEM > Marketing Cos’ > GET REAL ! FREE ? BUT Still within LIMITS ! U must Pay for necessary extra IF U need to Install ?
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![Under_Construction_anim-s1 Under_Construction_anim-s1](./Under_Construction_anim-s1_1.jpg) > ANY “Real AI”?
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some of Our Other Main OEM Suppliers !
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some of Our “KEY “ Products
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![CT Introduction2021>+ CT Introduction2021>+](./Intro_8.jpg)
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preferably Talk to US ! via e-mail > CHAT ? >spasmodic & NOT AI !
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but NOW “focus” on OUR -m 5 ! pages
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FlipHTML5 on Your Mobile ? & “responsive” ! > You may need to “Zoom’ ! ? “+Pinch & Rotate”but > OK ! ? as a preview ! THEN > any PC ! for THE Detail ! but Then FOCUS on -m5
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FlipHTML5 > LIBRARY
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Collaborative Projects WELCOME ? see here !
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NEW SEARCH 2023-10 > Try-It! “MS-Bing” SEARCH !
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* multiple Word Step Search Much Improved Over Google > & add “chromtech” OR “chromtech + Australia” to Your “Keywords
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& even BETTER for ALL of Us !
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> very “Useful” ! BUT NOT without IT’s “idiosynchrasies” TOO ! BUT it IS ! “AI” ???
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NOW ! > MS-Copilot & FREE !
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> extended Versions for MS Office APPs > even more AI BUT U need to “register” & THEN U must PAY for IT ? !
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Main Menu(s) see ( But with/ NO “Flyouts” )
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* Index-Extended to cover ALL HTMs & with Fly-outs > FLIP(S ( > All PDFs(DIR)
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