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& a HI-TECH “Resource” > 4 U !
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but see “Scams” > New”Phishes”
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CHROMTECH : Specialists in Chromatography Chromatography Fluid Transfer Products : & FOR ! All Your Laboratory Needs ! - Importers & Distributors in AUSTRALIA
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Chiral HPLC
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Applications
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Chromtech - Chiral Handbook 40p PDF
FlipPAGE H’Book
![Flip-A Flip-A](./Flip-A.jpg) PDF SEARCH works on text INDEX Only & NOT Pic chromatogrsms
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What is it! Separation of Optical isomers by HPLC. Often the critical moities in biologically active materials whether in bioscience or pharmaceuticals. This complex “mirror image” molecular separation is often given scant recognition by researchers primarily due to the difficulty in separating and analysing such raecemic mixtures. Let alone the difficulties of separating and purifing on a Preparative scale.
Chromtech chiral HPLC-columns for direct resolution of enantiomers - comprise 3 basic types
- CHIRAL-AGP ( USP chiral column L41 )
The CHIRAL-AGP column has extremely broad applicability. As can be seen from the applications a very large number of basic, acidic and nonprotolytic drugs and related compounds have been separated into enantiomers. The columns resolve chiral compounds containing primary-, secondary- or tertiary amino groups or quarternary ammonium groups, as well as chiral compounds containing carboxylic acid functions or weaker acidic groups as well as chiral nonprotolytes like alcohols, amides, sulphoxides, esters etc.
- CHIRAL-CBH
Cellobiohydrolase (CBH) is a very stable enzyme, which has been immobilized onto 5 micrometer spherical silica particles. This is a reversed-phase column, used for the direct separation of enantiomers. The column is preferantially used for the separation of enantiomers of basic drugs from many compound classes. CHIRAL-CBH separates preferentially compounds containing one or more basic nitrogen together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.)
- CHIRAL-HSA
The CHIRAL-HSA column is based on human serum albumin (HSA). The protein has been immobilized onto spherical 5 micrometer particles. Enantiomers of preferentially acidic compounds can be resolved directly, without derivatization. CHIRAL-HSA is especially suited for the resolution of weak and strong acids, zwitterionic and nonprotolytic compounds. However, basic compounds have also been resolved.
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Chiral Column Complete List
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Column Type
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CHIRAL-
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-DIM (mm)
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Analytical
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AGP CBH HSA
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150.4 100.4 50.4 150.3 100. 50.3
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Micro
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AGP CBH HSA
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150.2 100.2 50.2
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Semiprep
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AGP CBH HSA
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150.10 100.10
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Analytical Guard 2/pk
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AGP CBH HSA
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10.42 10.32 10.22
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Guard Column Holder
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for All
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CH10.3
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Guard Column Connector
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CON2
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Micro GC Connector
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CON4
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Column Dimensions
- Inner diameters 2, 3, 4, and 10 mm
- Lengths of 50, 100 and 150 mm.
- Guards with length of 10 mm and the inner diameters 2, 3 and 4 mm are also available
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Chiral columns for LC/MS
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Dimensions
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CHIRAL-AGP
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CHIRAL-CBH
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CHIRAL-HSA
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50x2.0 mm 100x2.0mm 150x2.0mm
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AGP50.2 AGP100.2 AGP150.2
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CBH50.2 CBH100.2 CBH150.2
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HSA50.2 HSA100.2 HSA150.2
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50x3.0mm 100x3.0mm 150x3.0mm
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AGP50.3 AGP100.3 AGP150.3
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CBH50.3 CBH100.3 CBH150.3
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HSA50.3 HSA100.3 HSA150.3
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50x4.0mm
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AGP60.4
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CBH50.4
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HSA50.4
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Extensive Application Notes are available . . . hundreds of separations often with huge “alpha” offering opportunies for massive scale up in column load capacities
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Application Areas At pharmaceutical companies, hospitals, universities and chemical industry CHIRAL-CBH is used for the analysis of enantiomeric purity and for bioanalysis. An interesting and fast growing area is isolation of pure enantiomers on semipreparative columns.
Mobile Phases The mobile phases are mixtures of phosphate or acetate buffers and organic solvents as 2-propanol or acetonitrile containing 50mM disodium EDTA. The retention and the enantioselectivity can be regulated by changes in pH, buffer concentration and organic modifier (nature and concentration).
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